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Method Article
Generation of Bioengineered Neuronal Organoids (BENOs) from human pluripotent stem cells
Maria-Patapia Zafeiriou
UMG Göttingen
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4604-4175
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This work is licensed under a CC BY 4.0 License. Read Full License
Here we present a new directed differentiation and maturation protocol to generate Bioengineered Neuronal Organoids (BENOs) comprised of complex networks of inhibitory and excitatory neurons as well as supporting glial cells (astrocytes and myelinating oligodendrocytes).
Keywords
Neuronal organoid, stem cell, neuronal network formation, glial cell, oligodendrocyte, plasticity, GABA polarity switch, long-term potentiation
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Associated Publications
Developmental GABA polarity switch and neuronal plasticity in Bioengineered Neuronal Organoids
by Maria-Patapia Zafeiriou, Guobin Bao, James Hudson, +6
Nature Communications (29 July, 2020)
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This is a preprint. It has not completed peer review.
Method Article
Generation of Bioengineered Neuronal Organoids (BENOs) from human pluripotent stem cells
Maria-Patapia Zafeiriou
UMG Göttingen
Corresponding Author
ORCiD: https://orcid.org/0000-0003-4604-4175
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 01 Aug, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we present a new directed differentiation and maturation protocol to generate Bioengineered Neuronal Organoids (BENOs) comprised of complex networks of inhibitory and excitatory neurons as well as supporting glial cells (astrocytes and myelinating oligodendrocytes).
Associated Publications
Developmental GABA polarity switch and neuronal plasticity in Bioengineered Neuronal Organoids
by Maria-Patapia Zafeiriou, Guobin Bao, James Hudson, +6
Nature Communications (29 July, 2020)