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Method Article
Stepwise Protocols for Converting Astrocytes to Neurons in vitro and in Mouse Brain by Depleting Polypyrimidine Tract Binding Protein PTB
Fu Xiang-Dong
UC, San Diego
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5499-8732
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This work is licensed under a CC BY 4.0 License. Read Full License
We recently develop an efficient single-step strategy to convert isolated mouse and human astrocytes into functional neurons by depleting the RNA binding protein PTB in isolated astrocytes in culture and directly in mouse brain. We show progressive conversion of astrocytes into new neurons that can innervate into endogenous neural circuits. Focusing on midbrain, we demonstrate efficient conversion of nigral astrocytes into dopaminergic neurons whose axons reconstruct the nigro-striatal circuit. Significantly, re-innervation of striatum is accompanied by restoration of dopamine levels and rescue of motor deficits. Herein, we present key methods employed in this study as stepwise protocols.
Keywords
Polypyrimidine tract binding protein, Parkinson’s disease, astrocytes, dopaminergic neurons, trans-differentiation, electrophysiologic recording, rotation and cylinder tests.
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This is a preprint. It has not completed peer review.
Method Article
Stepwise Protocols for Converting Astrocytes to Neurons in vitro and in Mouse Brain by Depleting Polypyrimidine Tract Binding Protein PTB
Fu Xiang-Dong
UC, San Diego
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5499-8732
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 25 Jun, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Neuroscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We recently develop an efficient single-step strategy to convert isolated mouse and human astrocytes into functional neurons by depleting the RNA binding protein PTB in isolated astrocytes in culture and directly in mouse brain. We show progressive conversion of astrocytes into new neurons that can innervate into endogenous neural circuits. Focusing on midbrain, we demonstrate efficient conversion of nigral astrocytes into dopaminergic neurons whose axons reconstruct the nigro-striatal circuit. Significantly, re-innervation of striatum is accompanied by restoration of dopamine levels and rescue of motor deficits. Herein, we present key methods employed in this study as stepwise protocols.