Stepwise Protocols for Converting Astrocytes to Neurons in vitro and in Mouse Brain by Depleting Polypyrimidine Tract Binding Protein PTB
We recently develop an efficient single-step strategy to convert isolated mouse and human astrocytes into functional neurons by depleting the RNA binding protein PTB in isolated astrocytes in culture and directly in mouse brain. We show progressive conversion of astrocytes into new neurons that can innervate into endogenous neural circuits. Focusing on midbrain, we demonstrate efficient conversion of nigral astrocytes into dopaminergic neurons whose axons reconstruct the nigro-striatal circuit. Significantly, re-innervation of striatum is accompanied by restoration of dopamine levels and rescue of motor deficits. Herein, we present key methods employed in this study as stepwise protocols.
Posted 25 Jun, 2020
Stepwise Protocols for Converting Astrocytes to Neurons in vitro and in Mouse Brain by Depleting Polypyrimidine Tract Binding Protein PTB
Posted 25 Jun, 2020
We recently develop an efficient single-step strategy to convert isolated mouse and human astrocytes into functional neurons by depleting the RNA binding protein PTB in isolated astrocytes in culture and directly in mouse brain. We show progressive conversion of astrocytes into new neurons that can innervate into endogenous neural circuits. Focusing on midbrain, we demonstrate efficient conversion of nigral astrocytes into dopaminergic neurons whose axons reconstruct the nigro-striatal circuit. Significantly, re-innervation of striatum is accompanied by restoration of dopamine levels and rescue of motor deficits. Herein, we present key methods employed in this study as stepwise protocols.
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