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Method Article
Lars M. Steinmetz
European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany; Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA; Stanford Genome Technology Center, Palo Alto, California 94304, USA
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3962-2865
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we provide a step-by-step protocol for targeted single-cell RNA-seq and targeted Perturb-seq, as reported in the linked publication Schraivogel et al. Nat Meth 2020. The protocol describes cell preparation using flow cytometry, single-cell droplet formation with 10X Genomics, and targeted 3’ single-cell RNA-seq library preparation for Illumina sequencing.
Keywords
single-cell, CRISPR, Cas9, CRISPRi, functional genomics, genetic screens, RNA sequencing
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The authors declare no competing financial interests.
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Method Article
Lars M. Steinmetz
European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany; Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA; Stanford Genome Technology Center, Palo Alto, California 94304, USA
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3962-2865
Version History
CURRENT STATUS: Posted
Version 1
Posted 02 Jun, 2020
Community comments: 1
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we provide a step-by-step protocol for targeted single-cell RNA-seq and targeted Perturb-seq, as reported in the linked publication Schraivogel et al. Nat Meth 2020. The protocol describes cell preparation using flow cytometry, single-cell droplet formation with 10X Genomics, and targeted 3’ single-cell RNA-seq library preparation for Illumina sequencing.
Figure 1
Figure 2
Figure 3
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