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Method Article
Sperm-seq wet lab protocol: sperm preparation and droplet-based sequencing library generation
Avery Davis Bell
Department of Genetics, Harvard Medical School; Program in Medical and Population Genetics, Broad Institute of MIT and Harvard
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1837-302X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Sperm-seq is a high-throughput, droplet-based single-sperm sequencing technology capable of generating thousands of cell-barcoded single-sperm sequencing libraries at one time. This protocol describes Sperm-seq library generation, featuring a full protocol for sperm preparation and suggestions for droplet-based sequencing methods from 10X Genomics to employ. Sperm preparation can be completed in half a day and the full protocol can be completed in 2-3 days, with several wait times and break points.
Keywords
sequencing, DNA sequencing, sequencing library generation, sperm, spermatozoa, single-cell sequencing, droplet-based sequencing, sperm nucleus decondensation
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This is a preprint. It has not completed peer review.
Method Article
Sperm-seq wet lab protocol: sperm preparation and droplet-based sequencing library generation
Avery Davis Bell
Department of Genetics, Harvard Medical School; Program in Medical and Population Genetics, Broad Institute of MIT and Harvard
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1837-302X
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 03 Jun, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Sperm-seq is a high-throughput, droplet-based single-sperm sequencing technology capable of generating thousands of cell-barcoded single-sperm sequencing libraries at one time. This protocol describes Sperm-seq library generation, featuring a full protocol for sperm preparation and suggestions for droplet-based sequencing methods from 10X Genomics to employ. Sperm preparation can be completed in half a day and the full protocol can be completed in 2-3 days, with several wait times and break points.