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Method Article
Avery Davis Bell
Department of Genetics, Harvard Medical School; Program in Medical and Population Genetics, Broad Institute of MIT and Harvard
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1837-302X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Sperm-seq is a high-throughput, droplet-based single-sperm sequencing technology capable of generating thousands of cell-barcoded single-sperm sequencing libraries at one time. This protocol describes Sperm-seq library generation, featuring a full protocol for sperm preparation and suggestions for droplet-based sequencing methods from 10X Genomics to employ. Sperm preparation can be completed in half a day and the full protocol can be completed in 2-3 days, with several wait times and break points.
Keywords
sequencing, DNA sequencing, sequencing library generation, sperm, spermatozoa, single-cell sequencing, droplet-based sequencing, sperm nucleus decondensation
A.D.B. and S.A.M. are inventors on a United States Provisional Patent application by the President and Fellows of Harvard College relating, at least in part, to this protocol. A.D.B. is an occasional consultant for Ohana Biosciences since October 2019.
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Version 1
Posted 03 Jun, 2020
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Genetics
Associated Publications
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Avery Davis Bell
Department of Genetics, Harvard Medical School; Program in Medical and Population Genetics, Broad Institute of MIT and Harvard
Corresponding Author
ORCiD: https://orcid.org/0000-0002-1837-302X
Version History
CURRENT STATUS: Posted
Version 1
Posted 03 Jun, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Sperm-seq is a high-throughput, droplet-based single-sperm sequencing technology capable of generating thousands of cell-barcoded single-sperm sequencing libraries at one time. This protocol describes Sperm-seq library generation, featuring a full protocol for sperm preparation and suggestions for droplet-based sequencing methods from 10X Genomics to employ. Sperm preparation can be completed in half a day and the full protocol can be completed in 2-3 days, with several wait times and break points.
A.D.B. and S.A.M. are inventors on a United States Provisional Patent application by the President and Fellows of Harvard College relating, at least in part, to this protocol. A.D.B. is an occasional consultant for Ohana Biosciences since October 2019.
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