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Method Article
Sangkyu Lee
Corresponding Author
Won Do Heo
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Numerous efforts have been made toward the goal of visualizing the spatiotemporal dynamics of single mRNA molecules, yet our capacity for precisely controlling their functions lags behind. Here, we present an optogenetic approach for manipulating the localization and translation of specific mRNAs in live cells. Our technique combines blue light-responsive protein-protein interactions with mRNA visualization modules to robustly and reversibly generate protein clusters that can trap specific mRNA molecules. This sequestration reduces the binding chance of mRNAs with ribosomes, thereby dramatically attenuating protein synthesis
Keywords
Optogenetics, Fluorescence microscopy, RNA imaging, RNA-protein interaction
Figure 1
The authors declare no competing financial interests.
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Associated Publications
Optogenetic control of mRNA localization and translation in live cells
by Na Yeon Kim, Sangkyu Lee, Jeonghye Yu, +4
Nature Cell Biology (17 February, 2020)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Sangkyu Lee
Corresponding Author
Won Do Heo
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 26 Mar, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Numerous efforts have been made toward the goal of visualizing the spatiotemporal dynamics of single mRNA molecules, yet our capacity for precisely controlling their functions lags behind. Here, we present an optogenetic approach for manipulating the localization and translation of specific mRNAs in live cells. Our technique combines blue light-responsive protein-protein interactions with mRNA visualization modules to robustly and reversibly generate protein clusters that can trap specific mRNA molecules. This sequestration reduces the binding chance of mRNAs with ribosomes, thereby dramatically attenuating protein synthesis
Figure 1
The authors declare no competing financial interests.
This is a list of supplementary files associated with this preprint. Click to download.
Loading...
Associated Publications
Optogenetic control of mRNA localization and translation in live cells
by Na Yeon Kim, Sangkyu Lee, Jeonghye Yu, +4
Nature Cell Biology (17 February, 2020)