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Method Article
Optogenetic control of mRNA localization and translation in live cells
Sangkyu Lee
Corresponding Author
Won Do Heo
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Numerous efforts have been made toward the goal of visualizing the spatiotemporal dynamics of single mRNA molecules, yet our capacity for precisely controlling their functions lags behind. Here, we present an optogenetic approach for manipulating the localization and translation of specific mRNAs in live cells. Our technique combines blue light-responsive protein-protein interactions with mRNA visualization modules to robustly and reversibly generate protein clusters that can trap specific mRNA molecules. This sequestration reduces the binding chance of mRNAs with ribosomes, thereby dramatically attenuating protein synthesis
Keywords
Optogenetics, Fluorescence microscopy, RNA imaging, RNA-protein interaction
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Associated Publications
Optogenetic control of mRNA localization and translation in live cells
by Na Yeon Kim, Sangkyu Lee, Jeonghye Yu, +4
Nature Cell Biology (17 February, 2020)
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This is a preprint. It has not completed peer review.
Method Article
Optogenetic control of mRNA localization and translation in live cells
Sangkyu Lee
Corresponding Author
Won Do Heo
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 26 Mar, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Numerous efforts have been made toward the goal of visualizing the spatiotemporal dynamics of single mRNA molecules, yet our capacity for precisely controlling their functions lags behind. Here, we present an optogenetic approach for manipulating the localization and translation of specific mRNAs in live cells. Our technique combines blue light-responsive protein-protein interactions with mRNA visualization modules to robustly and reversibly generate protein clusters that can trap specific mRNA molecules. This sequestration reduces the binding chance of mRNAs with ribosomes, thereby dramatically attenuating protein synthesis
Figure 1
Associated Publications
Optogenetic control of mRNA localization and translation in live cells
by Na Yeon Kim, Sangkyu Lee, Jeonghye Yu, +4
Nature Cell Biology (17 February, 2020)