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Method Article
Generation and rearing of axenic Aedes aegypti mosquitoes
Blaire Steven
Connecticut Agricultural Experiment Station
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5940-2432
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This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes the generation of axenic Aedes aegypti, which can be raised from larvae to adults in the absence of a microflora. The total protocol including the time taken to grow E. coli (used in one of the diets) takes two days to set up. The mosquitoes will develop normally with a slight developmental lag compared to conventionally raised mosquitoes (~1 week). Two larval diets are described, a liver:yeast (LY) extract diet and an LY diet supplemented with heat-inactivated E. coli. Axenic mosquitoes raised on food supplemented with heat-inactivated E. coli develop slightly faster than axenic mosquitoes raised without killed bacteria. This axenic system allows the manipulation of the mosquito microbiome and transitions the microbiome into a variable that can be controlled and manipulated in a mechanistic manner.
Keywords
Aedes aegypti, mosquito, axenic
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Associated Publications
Generation of axenic Aedes aegypti demonstrate live bacteria are not required for mosquito development
by Maria A. Correa, Brian Matusovsky, Doug E. Brackney, +1
Nature Communications (22 October, 2018)
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This is a preprint. It has not completed peer review.
Method Article
Generation and rearing of axenic Aedes aegypti mosquitoes
Blaire Steven
Connecticut Agricultural Experiment Station
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5940-2432
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 28 Nov, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes the generation of axenic Aedes aegypti, which can be raised from larvae to adults in the absence of a microflora. The total protocol including the time taken to grow E. coli (used in one of the diets) takes two days to set up. The mosquitoes will develop normally with a slight developmental lag compared to conventionally raised mosquitoes (~1 week). Two larval diets are described, a liver:yeast (LY) extract diet and an LY diet supplemented with heat-inactivated E. coli. Axenic mosquitoes raised on food supplemented with heat-inactivated E. coli develop slightly faster than axenic mosquitoes raised without killed bacteria. This axenic system allows the manipulation of the mosquito microbiome and transitions the microbiome into a variable that can be controlled and manipulated in a mechanistic manner.
Figure 1
Figure 2
Associated Publications
Generation of axenic Aedes aegypti demonstrate live bacteria are not required for mosquito development
by Maria A. Correa, Brian Matusovsky, Doug E. Brackney, +1
Nature Communications (22 October, 2018)