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Method Article
Single-cell multi-omic profiling of chromatin conformation and DNA methylation
Jesse R Dixon
Salk Institute for Biological Studies
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6273-2181
Joseph R Ecker
Salk Institute for Biological Studies
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5799-5895
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The ability to profile epigenomic features in single cells is facilitating the study of the variation in transcription regulation at the single cell level. Single cell methods have also facilitated the generation of cell-type resolved transcriptomic and epigenetic profiles of lineages derived from complex heterogeneous samples. However, integrating different epigenetic features remain challenging, as many current methods profile a single data type at at time. Furthermore, some epigenetic features, such as 3D genome organization, are intrinsically variable between single cells of the same lineage, so it remains unclear how well these methods may resolve cell-types from complex mixtures. Here we describe a method for profiling 3D genome organization and DNA methylation in single cells. This protocol accompanies Lee et al. (Nature Methods 2019) after peer review to aid potential users in applying the method to their own samples.
Keywords
Single Cell, 3D genome, DNA methylation, chromatin, epigenetics, genomics
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This is a preprint. It has not completed peer review.
Method Article
Single-cell multi-omic profiling of chromatin conformation and DNA methylation
Jesse R Dixon
Salk Institute for Biological Studies
Corresponding Author
ORCiD: https://orcid.org/0000-0002-6273-2181
Joseph R Ecker
Salk Institute for Biological Studies
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5799-5895
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 09 Sep, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The ability to profile epigenomic features in single cells is facilitating the study of the variation in transcription regulation at the single cell level. Single cell methods have also facilitated the generation of cell-type resolved transcriptomic and epigenetic profiles of lineages derived from complex heterogeneous samples. However, integrating different epigenetic features remain challenging, as many current methods profile a single data type at at time. Furthermore, some epigenetic features, such as 3D genome organization, are intrinsically variable between single cells of the same lineage, so it remains unclear how well these methods may resolve cell-types from complex mixtures. Here we describe a method for profiling 3D genome organization and DNA methylation in single cells. This protocol accompanies Lee et al. (Nature Methods 2019) after peer review to aid potential users in applying the method to their own samples.
Figure 1
Figure 2