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Method Article
Quantification of Translation efficiencies
Jörg Soppa
Goethe-University, Institute for Molecular Biosciences, Frankfurt am Main
Corresponding Author
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The determination of translational efficiencies for single transcripts of interest is described for E. coli and H. volcanii. In both cases, the transcript levels are quantified by Northern blot analyses, and the protein levels are quantified by reporter enzyme assays
Keywords
translational efficiency, translational regulation, northern blot, enzyme assay, reporter gene
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Associated Publications
Translational coupling via termination-reinitiation in archaea and bacteria
by Madeleine Huber, Guilhem Faure, Sebastian Laass, +6
Nature Communications (09 September, 2019)
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This is a preprint. It has not completed peer review.
Method Article
Quantification of Translation efficiencies
Jörg Soppa
Goethe-University, Institute for Molecular Biosciences, Frankfurt am Main
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 09 Sep, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The determination of translational efficiencies for single transcripts of interest is described for E. coli and H. volcanii. In both cases, the transcript levels are quantified by Northern blot analyses, and the protein levels are quantified by reporter enzyme assays
Associated Publications
Translational coupling via termination-reinitiation in archaea and bacteria
by Madeleine Huber, Guilhem Faure, Sebastian Laass, +6
Nature Communications (09 September, 2019)