Quantification of Translation efficiencies
The determination of translational efficiencies for single transcripts of interest is described for E. coli and H. volcanii. In both cases, the transcript levels are quantified by Northern blot analyses, and the protein levels are quantified by reporter enzyme assays
Posted 09 Sep, 2019
Quantification of Translation efficiencies
Posted 09 Sep, 2019
The determination of translational efficiencies for single transcripts of interest is described for E. coli and H. volcanii. In both cases, the transcript levels are quantified by Northern blot analyses, and the protein levels are quantified by reporter enzyme assays
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