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Method Article
Jörg Soppa
Goethe-University, Institute for Molecular Biosciences, Frankfurt am Main
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The determination of translational efficiencies for single transcripts of interest is described for E. coli and H. volcanii. In both cases, the transcript levels are quantified by Northern blot analyses, and the protein levels are quantified by reporter enzyme assays
Keywords
translational efficiency, translational regulation, northern blot, enzyme assay, reporter gene
The authors declare no competing financial interests.
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Posted 09 Sep, 2019
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Microbiology
Associated Publications
Translational coupling via termination-reinitiation in archaea and bacteria
by Madeleine Huber, Guilhem Faure, Sebastian Laass, +6
Nature Communications (09 September, 2019)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Jörg Soppa
Goethe-University, Institute for Molecular Biosciences, Frankfurt am Main
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 09 Sep, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Microbiology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The determination of translational efficiencies for single transcripts of interest is described for E. coli and H. volcanii. In both cases, the transcript levels are quantified by Northern blot analyses, and the protein levels are quantified by reporter enzyme assays
The authors declare no competing financial interests.
Loading...
Associated Publications
Translational coupling via termination-reinitiation in archaea and bacteria
by Madeleine Huber, Guilhem Faure, Sebastian Laass, +6
Nature Communications (09 September, 2019)