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Method Article
Sandra Iden
CECAD, University of Cologne
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2333-9827
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Dephosphorylation of epitopes is a common way of testing phospho-specificity of antibodies. Whereas it is a regularly used protocol for dephosphorylation in cell lysates, its use in immunohistochemistry has been limited. Here we describe a methodology to dephosphorylate adult murine tissues by using Antarctic Phosphatase, an alkaline phosphatase. This protocol is compatible with subsequent immunohistochemistry, making it suitable for validating phospho-immunoreactivity in tissues.
Keywords
tissue dephosphorylation, epitope dephosphorylation, alkaline phosphatase, antarctic phosphatase
No conflict of interests to declare.
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Associated Publications
Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
by Martim Dias Gomes, Soriba Letzian, Michael Saynisch, +1
Nature Communications (29 July, 2019)
Method Article
Sandra Iden
CECAD, University of Cologne
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2333-9827
Version History
CURRENT STATUS: Posted
Version 1
Posted 30 Jul, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Dephosphorylation of epitopes is a common way of testing phospho-specificity of antibodies. Whereas it is a regularly used protocol for dephosphorylation in cell lysates, its use in immunohistochemistry has been limited. Here we describe a methodology to dephosphorylate adult murine tissues by using Antarctic Phosphatase, an alkaline phosphatase. This protocol is compatible with subsequent immunohistochemistry, making it suitable for validating phospho-immunoreactivity in tissues.
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Associated Publications
Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
by Martim Dias Gomes, Soriba Letzian, Michael Saynisch, +1
Nature Communications (29 July, 2019)