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Method Article
Sandra Iden
CECAD, University of Cologne
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2333-9827
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Dephosphorylation of epitopes is a common way of testing phospho-specificity of antibodies. Whereas it is a regularly used protocol for dephosphorylation in cell lysates, its use in immunohistochemistry has been limited. Here we describe a methodology to dephosphorylate adult murine tissues by using Antarctic Phosphatase, an alkaline phosphatase. This protocol is compatible with subsequent immunohistochemistry, making it suitable for validating phospho-immunoreactivity in tissues.
Keywords
tissue dephosphorylation, epitope dephosphorylation, alkaline phosphatase, antarctic phosphatase
No conflict of interests to declare.
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Associated Publications
Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
by Martim Dias Gomes, Soriba Letzian, Michael Saynisch, +1
Nature Communications (29 July, 2019)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Sandra Iden
CECAD, University of Cologne
Corresponding Author
ORCiD: https://orcid.org/0000-0003-2333-9827
Version History
CURRENT STATUS: Posted
Version 1
Posted 30 Jul, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Dephosphorylation of epitopes is a common way of testing phospho-specificity of antibodies. Whereas it is a regularly used protocol for dephosphorylation in cell lysates, its use in immunohistochemistry has been limited. Here we describe a methodology to dephosphorylate adult murine tissues by using Antarctic Phosphatase, an alkaline phosphatase. This protocol is compatible with subsequent immunohistochemistry, making it suitable for validating phospho-immunoreactivity in tissues.
No conflict of interests to declare.
Loading...
Associated Publications
Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
by Martim Dias Gomes, Soriba Letzian, Michael Saynisch, +1
Nature Communications (29 July, 2019)