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Method Article
Spatio-temporal transcriptome construction of early mouse embryo with Geo-seq and Auto-seq
Guangdun Peng
CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Corresponding Author
Naihe Jing
State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences; University of Chinese Academy of Sciences
Corresponding Author
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The regionalization of cell fates heralding the formation of the basic body plan is conserved in vertebrate embryos, the mechanisms of which is instrumental for understanding embryonic programming and stem cell-based translational study. However, a comprehensive genome-wide molecular annotation of the mechanism that determines the lineage specification and cell fate decision has not been clarified. Here, we combined laser microdissection-based spatial transcriptome analysis (Geo-seq) and automated platform-based low-input RNA sequencing (Auto-seq), and generated a series of spatial transcriptome dataset that encompass mouse embryos from pre-gastrulation (E5.5) to late gastrulation (E7.5) on all germ layers in the embryo proper.
Keywords
Geo-seq, Auto-seq, Spatio-temporal transcriptome, Early embryo development
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Developmental biology
Associated Publications
Molecular architecture of lineage allocation and tissue organization in early mouse embryo
by Guangdun Peng, Shengbao Suo, Guizhong Cui, +10
Nature (07 August, 2019)
Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq
by Jun Chen, Shengbao Suo, Patrick PL Tam, +3
Nature Protocols (16 February, 2017)
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This is a preprint. It has not completed peer review.
Method Article
Spatio-temporal transcriptome construction of early mouse embryo with Geo-seq and Auto-seq
Guangdun Peng
CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
Corresponding Author
Naihe Jing
State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences; University of Chinese Academy of Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Aug, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Developmental biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The regionalization of cell fates heralding the formation of the basic body plan is conserved in vertebrate embryos, the mechanisms of which is instrumental for understanding embryonic programming and stem cell-based translational study. However, a comprehensive genome-wide molecular annotation of the mechanism that determines the lineage specification and cell fate decision has not been clarified. Here, we combined laser microdissection-based spatial transcriptome analysis (Geo-seq) and automated platform-based low-input RNA sequencing (Auto-seq), and generated a series of spatial transcriptome dataset that encompass mouse embryos from pre-gastrulation (E5.5) to late gastrulation (E7.5) on all germ layers in the embryo proper.
Associated Publications
Molecular architecture of lineage allocation and tissue organization in early mouse embryo
by Guangdun Peng, Shengbao Suo, Guizhong Cui, +10
Nature (07 August, 2019)
Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq
by Jun Chen, Shengbao Suo, Patrick PL Tam, +3
Nature Protocols (16 February, 2017)