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Method Article
Live imaging of mouse embryos during pre-implantation and peri-implantation development
Magdalena Zernicka-Goetz
University of Cambridge
Corresponding Author
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Live visualisation of embryo development is a powerful tool for scientists to understand how morphogenetic events shape the embryo. Here, we report on a culturing technique that allows live imaging of pre- and peri-implantation mouse embryos throughout the process of blastocyst to egg cylinder transition, enabling single cell tracking and delineation of the tissue dynamics accompanying this morphogenetic stage. At the same time, this protocol can be used for pharmacological manipulations of mouse embryos.
Keywords
Developmental Biology, Live imaging, Mouse embryogenesis
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Associated Publications
Morphogenesis of extra-embryonic tissues directs the remodelling of the mouse embryo at implantation
by Neophytos Christodoulou, Antonia Weberling, Douglas Strathdee, +3
Nature Communications (07 August, 2019)
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This is a preprint. It has not completed peer review.
Method Article
Live imaging of mouse embryos during pre-implantation and peri-implantation development
Magdalena Zernicka-Goetz
University of Cambridge
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 09 Aug, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Developmental biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Live visualisation of embryo development is a powerful tool for scientists to understand how morphogenetic events shape the embryo. Here, we report on a culturing technique that allows live imaging of pre- and peri-implantation mouse embryos throughout the process of blastocyst to egg cylinder transition, enabling single cell tracking and delineation of the tissue dynamics accompanying this morphogenetic stage. At the same time, this protocol can be used for pharmacological manipulations of mouse embryos.
Figure 1
Associated Publications
Morphogenesis of extra-embryonic tissues directs the remodelling of the mouse embryo at implantation
by Neophytos Christodoulou, Antonia Weberling, Douglas Strathdee, +3
Nature Communications (07 August, 2019)