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Protocol for Single-Cell RNA-Sequencing of Cryopreserved Human Liver Cells
Nadim Aizarani
MPI-IE (Freiburg)
Corresponding Author
Thomas F. Baumert
Inserm U1110 (Strasbourg)
Corresponding Author
Dominic Grün
MPI-IE (Freiburg)
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
This is a protocol describing how to perform scRNA-seq of cryopreserved human liver cells using the mCEL-Seq2 protocol as described in the following paper.
Title: A Human Liver Cell Atlas reveals Heterogeneity and Epithelial Progenitors
Authors: Nadim Aizarani, Antonio Saviano, Sagar, Laurent Mailly, Sarah Durand, Patrick Pessaux, Thomas F. Baumert, Dominic Grün
Journal: Nature
Keywords
Single-cell RNA-Sequencing (scRNA-seq), FACS, human liver cells, cryopreserved
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Associated Publications
A human liver cell atlas reveals heterogeneity and epithelial progenitors
by Nadim Aizarani, Antonio Saviano, Sagar, +6
Nature (10 July, 2019)
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This is a preprint. It has not completed peer review.
Protocol for Single-Cell RNA-Sequencing of Cryopreserved Human Liver Cells
Nadim Aizarani
MPI-IE (Freiburg)
Corresponding Author
Thomas F. Baumert
Inserm U1110 (Strasbourg)
Corresponding Author
Dominic Grün
MPI-IE (Freiburg)
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Jul, 2019
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This is a protocol describing how to perform scRNA-seq of cryopreserved human liver cells using the mCEL-Seq2 protocol as described in the following paper.
Title: A Human Liver Cell Atlas reveals Heterogeneity and Epithelial Progenitors
Authors: Nadim Aizarani, Antonio Saviano, Sagar, Laurent Mailly, Sarah Durand, Patrick Pessaux, Thomas F. Baumert, Dominic Grün
Journal: Nature
Associated Publications
A human liver cell atlas reveals heterogeneity and epithelial progenitors
by Nadim Aizarani, Antonio Saviano, Sagar, +6
Nature (10 July, 2019)