Assessment of macro, trace and toxic element intake from rice: differences between cultivars, pigmented and non-pigmented rice

doi:10.21203/rs.3.pex-2621/v1

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Assessment of macro, trace and toxic element intake from rice: differences between cultivars, pigmented and non-pigmented rice

https://doi.org/10.21203/rs.3.pex-2621/v1

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Pigmented and non-pigmented rice varieties (grown in different areas) were collected in China, Yunnan, to investigate the content of macro-, trace elements and potentially toxic elements (PTEs), and to assess the health risk associated with dietary intake. The order of elemental concentrations in rice was Mn > Zn > Fe > Cu > Se for trace elements, P > K > Mg > Ca > Na for macro elements, and Cr > As > Cd for PTEs. Rice with a high concentration of essential elements also associated with a high content of PTEs. In addition, higher content of Cr, Mn and Na were found in pigmented rice. The health risk assessment showed that the daily intake of all elements was below the tolerable limit (UL). Moreover the intake of Fe, Zn and Se was far from sufficient for the nutrient requirement. The PTEs in rice dominated the health risk. Of concern is that this rice consumption is likely to contribute to carcinogenic risks in the long term and that adults are at higher health risk from pigmented rice compared to non-pigmented rice. This study confirms that the lack of essential micronutrients in rice and the health risk associated with rice diets should remain a concern.

Biochemistry

rice

essential micronutrients

toxic elements

pigmented rice

health risk

Quality Standards and Testing Technology Reaearch Institute, Yunnan Academy of Agricultural Sciences

nitric acid, perchloric acid (10:1) (ACS reagent, > 90%);multi-element standard solution (SGB-YYA230011);certified wheat flour reference material (GBW (E) 100493)

certified wheat flour reference material (GBW (E) 100493)

Total concentrations of macro, trace, and toxic elements were analyzed by the closed wet digestion method. Approximately 1 g ( accurate to 0.001g ) of the dry sample is weighed into polytetrafluoroethylene digestion vessel, then 10 ml of a mixed solution of nitric acid and perchloric acid (10:1) (ACS reagent, > 90%) is added, then heated from 25 °C to 95 °C for 45 minutes and held at 95 °C for 90 minutes using a hot plate until the digestion solution is colorless and transparent or slightly yellow, removed and cooled to room temperature, then diluted to 25 ml with water and shaken well. All samples were digested in triplicate during rice component metal analysis, and a blank test was performed simultaneously.

The digested solution was analysed for the content of Pb, Cd, Cr, As, Hg, Cu, Zn, Fe, Ca, K, Mg, Mn, Na, P, and Se by inductively coupled plasma mass spectrometry (ICP-MS) (PerkinElmer SCIEX ELAN DRC-e, Massachusetts, USA). The specific working parameters were set as follows: Automatic lens mode on, peak-hopping measurement mode, dwell time of 50 ms, 10 sweeps/measurement, 1500 W power, sample feed rate of 0.87 ml min^-1. The concentrations of macro and trace minerals and toxic metals in the samples were expressed in mg kg^-1 and μg kg^-1 on a dry weight basis, respectively. The elemental content of the samples was quantified using the external standard method of multi-element standard solution (SGB-YYA230011). A rigorous quality control programme was also implemented to ensure the accuracy of the analytical results. This included blank samples, certified wheat flour reference material (GBW (E) 100493) and the mean blank value was subtracted from the measured values before the result was calculated.

The elemental content of the samples was quantified using the external standard method of multi-element standard solution (SGB-YYA230011). A rigorous quality control programme was also implemented to ensure the accuracy of the analytical results. This included blank samples, certified wheat flour reference material (GBW (E) 100493) and the mean blank value was subtracted from the measured values before the result was calculated.

12h

(1) analyze the rice concentration of PTEs, macro minerals (Na, P, K, Mg and Ca) and trace elements (Mn, Zn, Fe, Cu and Se ) from Yunnan, China; (2) rice element differences between rice production area, pigmented and non-pigmented; (3) evaluate the potential health risks of high quality rice commonly used in the local market. The results would be valuable for the adaptation of suitable growing areas and varieties, useful for the control of heavy metals and the enrichment of trace elements.

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We would like to thank Yunnan Province Science and Technology Department for supplying the financial support.

This work was supported by the Major Science and Technology Projects in Yunnan Province, China under Grant No. 202102AE090051.

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Assessment of macro, trace and toxic element intake from rice: differences between cultivars, pigmented and non-pigmented rice

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Assessment of macro, trace and toxic element intake from rice: differences between cultivars, pigmented and non-pigmented rice

Status:

Journal Publication

Version 1

Abstract

Figures

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Time Taken

Anticipated Results

References

Acknowledgements

Supplementary Files

Associated Publications

Status:

Journal Publication

Version 1

Privacy Policy

Terms of Service

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