Antibodies can opsonise pathogens and promote opsonic phagocytosis by immune cells (e.g. neutrophils, monocytes, macrophages). High levels of antibodies capable of inducing opsonic phagocytosis have been associated with protective immunity in several diseases, including malaria. This protocol measures phagocytosis by neutrophils of either antibody-opsonised P. falciparum merozoites or antigen-coated beads. This approach is valuable to assess the role of antibodies in in immunity to malaria, either naturally acquired or vaccine-induced. Antigen-coated beads can be a useful alternative to using whole malaria parasites (or other organisms), and also have the advantage of enabling assays that quantify activity with specific antigenic targets. These assays can be used with a range of different malaria parasite stages including, merozoites, sporozoites, and infected erythrocytes. This protocol describes the use of neutrophils as the phagocyte, but can be adapted to use monocytes and macrophages, or phagocytic cell lines such as THP-1.