IgG can opsonise pathogens and mediate binding to Fcγ-receptors (FcγRs) expressed by various innate immune cells to promote antibody-mediated pathogen clearance via mechanisms including opsonic phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). High levels of IgG that bind FcγRs has been associated with protection from malaria. This assay measures FcγR-binding by antigen-specific IgG using recombinant FcγRs in a plate-based method which is suitable for high throughput. The method is suitable for using recombinant proteins or whole malaria parasites in the assays. FcγRIIa and FcγRIIIa/b are low affinity receptors and need to be used as ectodomain dimers in assays in order to achieve good activity in the assays. FcγRI can be used as a monomeric protein.