A growing system closer to the natural conditions for studying root growth and response of Arabidopsis thaliana
In natural environments, shoot was exposed to sunlight but roots are not directly exposed to light, being buried deep down in the soil and grow in darkness without sucrose addition. However, most current research in root biology has been carried out by growing the Arabidopsis root system in the presence of light and sucrose-added medium since root development is typically studied in traditional agar-plate culture system (TPG, traditional plant-growing). Here, we present a protocol for improved agar-plate culture system (IPG, improved plant-growing), allows the in vitro cultivation of Arabidopsis with the aerial part growing in normal light/dark photoperiod while roots grow in darkness without sucrose addition. IPG provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. We also provide methods for key steps all the way through to seedlings growth.We found that the primary root lengths in TPG were significantly longer than that in IPG, and the root elongation growth is dependent on auxin-triggered acidification of the root apoplast of Arabidopsis . With this protocol, a better understanding of the mechanisms involved in root growth and responses of plants can be easily characterized.
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I wish to use a system like this for Arabidopsis lyrata, a close relative of A. thaliana. What is to keep the media from getting contaminated with fungi when there are oblong holes in the plates? And, where does one purchase these plates, or does one have to make them from standard plates? Thank you.
Posted 08 Apr, 2019
A growing system closer to the natural conditions for studying root growth and response of Arabidopsis thaliana
Posted 08 Apr, 2019
In natural environments, shoot was exposed to sunlight but roots are not directly exposed to light, being buried deep down in the soil and grow in darkness without sucrose addition. However, most current research in root biology has been carried out by growing the Arabidopsis root system in the presence of light and sucrose-added medium since root development is typically studied in traditional agar-plate culture system (TPG, traditional plant-growing). Here, we present a protocol for improved agar-plate culture system (IPG, improved plant-growing), allows the in vitro cultivation of Arabidopsis with the aerial part growing in normal light/dark photoperiod while roots grow in darkness without sucrose addition. IPG provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. We also provide methods for key steps all the way through to seedlings growth.We found that the primary root lengths in TPG were significantly longer than that in IPG, and the root elongation growth is dependent on auxin-triggered acidification of the root apoplast of Arabidopsis . With this protocol, a better understanding of the mechanisms involved in root growth and responses of plants can be easily characterized.
Figure 1
Figure 2
Figure 3
Figure 4
I wish to use a system like this for Arabidopsis lyrata, a close relative of A. thaliana. What is to keep the media from getting contaminated with fungi when there are oblong holes in the plates? And, where does one purchase these plates, or does one have to make them from standard plates? Thank you.
There is no sucrose included in the medium container of IPG, therefore, the medium is rarely contaminated by fungi. These IPG plates are custom-made. You can also refer to the 'Xu et al. 2013. An improved agar-plate method for studying root growth and response of Arabidopsis thaliana. Scientific Reports.3, 1273 '.
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Ying Li
replied on 16 September, 2020
There is no sucrose included in the medium container of IPG, therefore, the medium is rarely contaminated by fungi. These IPG plates are custom-made. You can also refer to the 'Xu et al. 2013. An improved agar-plate method for studying root growth and response of Arabidopsis thaliana. Scientific Reports.3, 1273 '.