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A growing system closer to the natural conditions for studying root growth and response of Arabidopsis thaliana
Weifeng Xu
Fujian Agriculture and Forestry University
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
In natural environments, shoot was exposed to sunlight but roots are not directly exposed to light, being buried deep down in the soil and grow in darkness without sucrose addition. However, most current research in root biology has been carried out by growing the Arabidopsis root system in the presence of light and sucrose-added medium since root development is typically studied in traditional agar-plate culture system (TPG, traditional plant-growing). Here, we present a protocol for improved agar-plate culture system (IPG, improved plant-growing), allows the in vitro cultivation of Arabidopsis with the aerial part growing in normal light/dark photoperiod while roots grow in darkness without sucrose addition. IPG provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. We also provide methods for key steps all the way through to seedlings growth.We found that the primary root lengths in TPG were significantly longer than that in IPG, and the root elongation growth is dependent on auxin-triggered acidification of the root apoplast of Arabidopsis . With this protocol, a better understanding of the mechanisms involved in root growth and responses of plants can be easily characterized.
Keywords
Arabidopsis, auxin, growth, pH, root
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Bernadette M Roche
commented on 27 May, 2020
I wish to use a system like this for Arabidopsis lyrata, a close relative of A. thaliana. What is to keep the media from getting contaminated with fungi when there are oblong holes in the plates? And, where does one purchase these plates, or does one have to make them from standard plates? Thank you.
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Associated Publications
An improved agar-plate method for studying root growth and response of Arabidopsis thaliana
by Weifeng Xu, Guochang Ding, Ken Yokawa, +6
Scientific Reports (14 February, 2013)
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This is a preprint. It has not completed peer review.
A growing system closer to the natural conditions for studying root growth and response of Arabidopsis thaliana
Weifeng Xu
Fujian Agriculture and Forestry University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Apr, 2019
Community comments: 2
Metrics
Comments: 2
HTML Views: ...
Subject Areas
Plant sciences
License:
This work is licensed under a CC BY 4.0 License. Read Full License
In natural environments, shoot was exposed to sunlight but roots are not directly exposed to light, being buried deep down in the soil and grow in darkness without sucrose addition. However, most current research in root biology has been carried out by growing the Arabidopsis root system in the presence of light and sucrose-added medium since root development is typically studied in traditional agar-plate culture system (TPG, traditional plant-growing). Here, we present a protocol for improved agar-plate culture system (IPG, improved plant-growing), allows the in vitro cultivation of Arabidopsis with the aerial part growing in normal light/dark photoperiod while roots grow in darkness without sucrose addition. IPG provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. We also provide methods for key steps all the way through to seedlings growth.We found that the primary root lengths in TPG were significantly longer than that in IPG, and the root elongation growth is dependent on auxin-triggered acidification of the root apoplast of Arabidopsis . With this protocol, a better understanding of the mechanisms involved in root growth and responses of plants can be easily characterized.
Figure 1
Figure 2
Figure 3
Figure 4
Bernadette M Roche
commented on 27 May, 2020
I wish to use a system like this for Arabidopsis lyrata, a close relative of A. thaliana. What is to keep the media from getting contaminated with fungi when there are oblong holes in the plates? And, where does one purchase these plates, or does one have to make them from standard plates? Thank you.
View 1 reply
Ying Li
replied on 16 September, 2020
There is no sucrose included in the medium container of IPG, therefore, the medium is rarely contaminated by fungi. These IPG plates are custom-made. You can also refer to the 'Xu et al. 2013. An improved agar-plate method for studying root growth and response of Arabidopsis thaliana. Scientific Reports.3, 1273 '.
Associated Publications
An improved agar-plate method for studying root growth and response of Arabidopsis thaliana
by Weifeng Xu, Guochang Ding, Ken Yokawa, +6
Scientific Reports (14 February, 2013)
Ying Li
replied on 16 September, 2020
There is no sucrose included in the medium container of IPG, therefore, the medium is rarely contaminated by fungi. These IPG plates are custom-made. You can also refer to the 'Xu et al. 2013. An improved agar-plate method for studying root growth and response of Arabidopsis thaliana. Scientific Reports.3, 1273 '.