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Weifeng Xu
Fujian Agriculture and Forestry University
Corresponding Author
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In natural environments, shoot was exposed to sunlight but roots are not directly exposed to light, being buried deep down in the soil and grow in darkness without sucrose addition. However, most current research in root biology has been carried out by growing the Arabidopsis root system in the presence of light and sucrose-added medium since root development is typically studied in traditional agar-plate culture system (TPG, traditional plant-growing). Here, we present a protocol for improved agar-plate culture system (IPG, improved plant-growing), allows the in vitro cultivation of Arabidopsis with the aerial part growing in normal light/dark photoperiod while roots grow in darkness without sucrose addition. IPG provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. We also provide methods for key steps all the way through to seedlings growth.We found that the primary root lengths in TPG were significantly longer than that in IPG, and the root elongation growth is dependent on auxin-triggered acidification of the root apoplast of Arabidopsis . With this protocol, a better understanding of the mechanisms involved in root growth and responses of plants can be easily characterized.
Keywords
Arabidopsis, auxin, growth, pH, root
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Posted 08 Apr, 2019
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Plant sciences
Associated Publications
An improved agar-plate method for studying root growth and response of Arabidopsis thaliana
by Weifeng Xu, Guochang Ding, Ken Yokawa, +6
Scientific Reports (14 February, 2013)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Weifeng Xu
Fujian Agriculture and Forestry University
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 08 Apr, 2019
Community comments: 2
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Plant sciences
License:
This work is licensed under a CC BY 4.0 License. Read Full License
In natural environments, shoot was exposed to sunlight but roots are not directly exposed to light, being buried deep down in the soil and grow in darkness without sucrose addition. However, most current research in root biology has been carried out by growing the Arabidopsis root system in the presence of light and sucrose-added medium since root development is typically studied in traditional agar-plate culture system (TPG, traditional plant-growing). Here, we present a protocol for improved agar-plate culture system (IPG, improved plant-growing), allows the in vitro cultivation of Arabidopsis with the aerial part growing in normal light/dark photoperiod while roots grow in darkness without sucrose addition. IPG provides an efficient system for cultivating a large number of seedlings and easily characterizing root architecture in the dark. We also provide methods for key steps all the way through to seedlings growth.We found that the primary root lengths in TPG were significantly longer than that in IPG, and the root elongation growth is dependent on auxin-triggered acidification of the root apoplast of Arabidopsis . With this protocol, a better understanding of the mechanisms involved in root growth and responses of plants can be easily characterized.
Figure 1
Figure 2
Figure 3
Figure 4
no
Loading...
Associated Publications
An improved agar-plate method for studying root growth and response of Arabidopsis thaliana
by Weifeng Xu, Guochang Ding, Ken Yokawa, +6
Scientific Reports (14 February, 2013)