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Method Article
Song Ja Kim
Kongju National University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6380-256X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Chondrocyte dedifferentiation is a cellular phenomenon in which chondrocyte loses its chondrogenic characteristics and ability to synthesize extracellular matrix. These conditions commonly reported in different cartilage degenerative diseases. The methods used to induce dedifferentiation in cell models of chondrocytes are often time consuming or require expensive reagents. Here in our protocol, we describe the utility of using 2-deoxy-D-glucose as a dedifferentiating agent for rabbit chondrocytes for studying dedifferentiation process.
Keywords
Chondrocytes, Dedifferentiation, Articular Cartilages, 2-deoxy-D-glucose
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Cell Biology
Associated Publications
2-Deoxy-D-glucose regulates dedifferentiation through β-catenin pathway in rabbit articular chondrocytes
by Seon-Mi Yu, Hyun Ah Kim, Song-Ja Kim
Experimental and Molecular Medicine (21 July, 2010)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Song Ja Kim
Kongju National University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6380-256X
Version History
CURRENT STATUS: Posted
Version 1
Posted 16 Sep, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Chondrocyte dedifferentiation is a cellular phenomenon in which chondrocyte loses its chondrogenic characteristics and ability to synthesize extracellular matrix. These conditions commonly reported in different cartilage degenerative diseases. The methods used to induce dedifferentiation in cell models of chondrocytes are often time consuming or require expensive reagents. Here in our protocol, we describe the utility of using 2-deoxy-D-glucose as a dedifferentiating agent for rabbit chondrocytes for studying dedifferentiation process.
Figure 1
Figure 2
Figure 3
Figure 4
Loading...
Associated Publications
2-Deoxy-D-glucose regulates dedifferentiation through β-catenin pathway in rabbit articular chondrocytes
by Seon-Mi Yu, Hyun Ah Kim, Song-Ja Kim
Experimental and Molecular Medicine (21 July, 2010)