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Mouse intestinal organoid time-course experiments from single cells
Prisca Liberali
Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Corresponding Author
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Organoids recapitulate the self-organizing capacity of stem cells and the tissue organization of the original organ in a controlled and trackable environment. Intestinal organoids, in particular, can develop from a single cell into a fully-grown structure that contains most of the cell types, patterns, and morphogenetic properties of the adult intestine. Here we present a protocol for high-throughput organoid culture in multi-well plate format combined with high content immunofluorescence imaging and RNA extraction. Our protocol allows recording and analysis of thousands of organoids during several days of development.
Keywords
organoid, self-organisation, high throughput microscopy, multiplexed immunofluorescence imaging, RNA extraction
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This is a preprint. It has not completed peer review.
Mouse intestinal organoid time-course experiments from single cells
Prisca Liberali
Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 26 Apr, 2019
No community comments so far
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Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Organoids recapitulate the self-organizing capacity of stem cells and the tissue organization of the original organ in a controlled and trackable environment. Intestinal organoids, in particular, can develop from a single cell into a fully-grown structure that contains most of the cell types, patterns, and morphogenetic properties of the adult intestine. Here we present a protocol for high-throughput organoid culture in multi-well plate format combined with high content immunofluorescence imaging and RNA extraction. Our protocol allows recording and analysis of thousands of organoids during several days of development.