An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Colenso M. Speer
University of Maryland - College Park
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3076-7072
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D STORM image quality by reducing tissue scattering, photobleaching, and optical distortions associated with deep imaging. This approach can be extended for use on other tissue types enabling life scientists to perform volumetric super-resolution imaging in diverse biological models.
For a detailed application of this protocol, please refer to Sigal et al., 2015.
Keywords
Super-resolution microscopy, Neural circuits, synapses
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
This is a list of supplementary files associated with this preprint. Click to download.
- VolumetricsuperresolutionimagingbyserialultrasectioningandSTochasticOpticalReconstructionMicroscopySTORMProtocol.pdf
Volumetric super-resolution imaging by serial ultrasectioning and STochastic Optical Reconstruction Microscopy (STORM) Protocol
- ReagentRecipeTable.pdf
Reagent Recipe Table
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 04 Aug, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Neuroscience
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Colenso M. Speer
University of Maryland - College Park
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3076-7072
Version History
CURRENT STATUS: Posted
Version 1
Posted 04 Aug, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Neuroscience
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we present a protocol for collecting large-volume, four-color, single-molecule localization imaging data from neural tissue. We have applied this technique to map the location and identities of chemical synapses across whole cells in mouse retinae. Our sample preparation approach improves 3D STORM image quality by reducing tissue scattering, photobleaching, and optical distortions associated with deep imaging. This approach can be extended for use on other tissue types enabling life scientists to perform volumetric super-resolution imaging in diverse biological models.
For a detailed application of this protocol, please refer to Sigal et al., 2015.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
This is a list of supplementary files associated with this preprint. Click to download.
- VolumetricsuperresolutionimagingbyserialultrasectioningandSTochasticOpticalReconstructionMicroscopySTORMProtocol.pdf
Volumetric super-resolution imaging by serial ultrasectioning and STochastic Optical Reconstruction Microscopy (STORM) Protocol
- ReagentRecipeTable.pdf
Reagent Recipe Table
Loading...