Social interactions between rodents is commonly evaluated using setups that enable restricted interactions between two rodents. However, such interactions are most often evaluated only for the animal defined as subject, but not for the social stimuli, which are usually located in a specific compartment. In most cases, behavioral quantification is done using a single video camera that allows visualizing the subject, but not the stimuli. To assess stimuli behavior in such experiments, we constructed a movement-monitoring system, comprising an array of piezoelectric sensors located at the floor of a small triangular chamber to which the social stimulus is allocated. The system and quantification methodology described in the following protocol, together with a camera recorded simultaneously, enabled us to simultaneously evaluate the movements of one or two social stimuli and the subject's investigatory behavior correlated with it.

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Posted 02 Aug, 2021
Posted 02 Aug, 2021
Social interactions between rodents is commonly evaluated using setups that enable restricted interactions between two rodents. However, such interactions are most often evaluated only for the animal defined as subject, but not for the social stimuli, which are usually located in a specific compartment. In most cases, behavioral quantification is done using a single video camera that allows visualizing the subject, but not the stimuli. To assess stimuli behavior in such experiments, we constructed a movement-monitoring system, comprising an array of piezoelectric sensors located at the floor of a small triangular chamber to which the social stimulus is allocated. The system and quantification methodology described in the following protocol, together with a camera recorded simultaneously, enabled us to simultaneously evaluate the movements of one or two social stimuli and the subject's investigatory behavior correlated with it.

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