Preparation of fluorescent beads under C. elegans for fluorescence microscopy with adaptive optics

doi:10.21203/rs.3.pex-1458/v1

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Method Article

Ruizhe Lin, Edward T. Kipreos, Peter Kner

Ruizhe Lin

University of Georgia

ORCiD: https://orcid.org/0000-0003-1897-1019

Edward T. Kipreos

University of Georgia

ORCiD: https://orcid.org/0000-0001-9650-3558

Peter Kner

University of Georgia

Corresponding Author

ORCiD: https://orcid.org/0000-0001-7555-5180

DOI:

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We describe a protocol for the preparation of a sample of fluorescent beads under C. elegans. This type of sample is useful for research into the use of adaptive optics to correct sample-induced optical aberrations in fluorescence microscopy.

Keywords

Fluorescent beads, C. elegans, fluorescence microscopy, adaptive optics

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An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Ruizhe Lin, Edward T. Kipreos, Peter Kner

Ruizhe Lin

University of Georgia

ORCiD: https://orcid.org/0000-0003-1897-1019

Edward T. Kipreos

University of Georgia

ORCiD: https://orcid.org/0000-0001-9650-3558

Peter Kner

University of Georgia

Corresponding Author

ORCiD: https://orcid.org/0000-0001-7555-5180

Version History

CURRENT STATUS: Posted

Version 1

Posted 30 Apr, 2021

No community comments so far

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An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Ruizhe Lin, Edward T. Kipreos, Peter Kner

Ruizhe Lin

University of Georgia

ORCiD: https://orcid.org/0000-0003-1897-1019

Edward T. Kipreos

University of Georgia

ORCiD: https://orcid.org/0000-0001-9650-3558

Peter Kner

University of Georgia

Corresponding Author

ORCiD: https://orcid.org/0000-0001-7555-5180

DOI:

10.21203/rs.3.pex-1458/v1

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

Fluorescent beads, C. elegans, fluorescence microscopy, adaptive optics

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Time Taken

Anticipated Results

References

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CURRENT STATUS: Posted

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Biological techniques

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Ruizhe Lin, Edward T. Kipreos, Peter Kner

Ruizhe Lin

University of Georgia

ORCiD: https://orcid.org/0000-0003-1897-1019

Edward T. Kipreos

University of Georgia

ORCiD: https://orcid.org/0000-0001-9650-3558

Peter Kner

University of Georgia

Corresponding Author

ORCiD: https://orcid.org/0000-0001-7555-5180

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CURRENT STATUS: Posted

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Subject Areas

Biological techniques

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DOI:

10.21203/rs.3.pex-1458/v1

Download PDF

License

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Declarations

Abstract

Introduction

Reagents

Equipment

Procedure