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Method Article
Yun Bai
Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University Health Science Center, Peking University, Beijing 100191, China.
Corresponding Author
Jun Xu
Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University Health Science Center, Peking University, Beijing 100191, China.
Corresponding Author
Hongkui Deng
Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Human extended pluripotent stem (EPS) cells have shown great applicative potentials in basic and translational research. One prerequisite for the wide applications of EPS cells is the development of a xeno-free culture system for maintain these cells in vitro. Here we report a protocol for culturing and generating human EPS cells using a chemically defined and xeno-free culture system.
Keywords
Human extended pluripotent stem cells, Xeno-free, Chemically defined medium
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Posted 26 May, 2021
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Associated Publications
Chemically defined and xeno-free culture condition for human extended pluripotent stem cells
by Bei Liu, Shi Chen, Yaxing Xu, +13
Nature Communications (21 May, 2021)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Yun Bai
Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University Health Science Center, Peking University, Beijing 100191, China.
Corresponding Author
Jun Xu
Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University Health Science Center, Peking University, Beijing 100191, China.
Corresponding Author
Hongkui Deng
Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, and the MOE Key Laboratory of Cell Proliferation and Differentiation, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100191, China.
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 26 May, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Stem cells
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Human extended pluripotent stem (EPS) cells have shown great applicative potentials in basic and translational research. One prerequisite for the wide applications of EPS cells is the development of a xeno-free culture system for maintain these cells in vitro. Here we report a protocol for culturing and generating human EPS cells using a chemically defined and xeno-free culture system.
Loading...
Associated Publications
Chemically defined and xeno-free culture condition for human extended pluripotent stem cells
by Bei Liu, Shi Chen, Yaxing Xu, +13
Nature Communications (21 May, 2021)