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Method Article
Julián Andrés Castillo Vargas
Universidade Federal Rural da Amazônia
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5163-5127
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The development of novel methods to estimate the number of protein genetic variants of caseins (CN) and whey proteins in buffalo’s milk is necessary to optimize the technological and nutritional properties of dairy derivative products from this species. The literature has shown that buffalo’s milk CN and whey proteins can be successfully quantified using RP-HPLC-DAD. However, pure standards of genetic variants of CN and whey proteins in buffalo’s milk are not commercially available. This condition makes necessary their isolation and purification by preparative chromatography, which is difficult and requires specialized equipment. Thus, we proposed a protocol to quantify different casein genetic variants and major whey proteins in the milk of water buffaloes, using the combination of measurements derived from RP-HPLC-DAD and NIRS techniques. Our data showed estimates of casein variants and whey proteins similar to those reported by the classical method RP-HPLC-DAD that uses purified proteins and calibration curves.
Keywords
Casein, Liquid-chromatography, Milk, Ruminant, Whey protein
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
- Sampleofcalculationprotocol.xlsx
Datasheet for protein concentration calculations
- Table1.pdf
Table 1. Chromatographic elution gradient
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CURRENT STATUS: Posted
Version 1
Posted 06 Apr, 2021
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Julián Andrés Castillo Vargas
Universidade Federal Rural da Amazônia
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5163-5127
Version History
CURRENT STATUS: Posted
Version 1
Posted 06 Apr, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The development of novel methods to estimate the number of protein genetic variants of caseins (CN) and whey proteins in buffalo’s milk is necessary to optimize the technological and nutritional properties of dairy derivative products from this species. The literature has shown that buffalo’s milk CN and whey proteins can be successfully quantified using RP-HPLC-DAD. However, pure standards of genetic variants of CN and whey proteins in buffalo’s milk are not commercially available. This condition makes necessary their isolation and purification by preparative chromatography, which is difficult and requires specialized equipment. Thus, we proposed a protocol to quantify different casein genetic variants and major whey proteins in the milk of water buffaloes, using the combination of measurements derived from RP-HPLC-DAD and NIRS techniques. Our data showed estimates of casein variants and whey proteins similar to those reported by the classical method RP-HPLC-DAD that uses purified proteins and calibration curves.
Figure 1
This is a list of supplementary files associated with this preprint. Click to download.
- Sampleofcalculationprotocol.xlsx
Datasheet for protein concentration calculations
- Table1.pdf
Table 1. Chromatographic elution gradient
Loading...