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Method Article
Hua Zhang
China Agricultural University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The communications between oocyte and granulosa cells are essential for ovarian folliculogenesis in mammals, and the communicating structures in the ZP had been widely reported. However, there was lack of direct approach to visualize these structures in a 3D model with a high-resolution. Here, we present a protocol to image the structures which derived from oocytes by combining endogenous fluorescent mouse models with a high-resolution spinning disc-confocal system in details
Keywords
oocyte, granulosa cell, high-resolution 3D imaging, oocyte microvilli
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Cell Biology
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Hua Zhang
China Agricultural University
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 16 Mar, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The communications between oocyte and granulosa cells are essential for ovarian folliculogenesis in mammals, and the communicating structures in the ZP had been widely reported. However, there was lack of direct approach to visualize these structures in a 3D model with a high-resolution. Here, we present a protocol to image the structures which derived from oocytes by combining endogenous fluorescent mouse models with a high-resolution spinning disc-confocal system in details
Figure 1
Figure 2
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