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Method Article
Shaun D. McCullough
US Environmental Protection Agency
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6660-346X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol is intended for culture of primary human tracheobronchial epithelial cells (pHBEC) obtained by brush biopsy during clinical bronchoscopy, or purchased commercially, using Lonza-based medium.
Note: This is a historical protocol. At the time of publication, this protocol has been superseded by a different version in the McCullough lab; however, it is being published to support the transparency and reproducibility of other studies by which it is referenced.
Disclaimer: The information presented here has been reviewed and approved for publication by the US Environmental Protection Agency do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.
Keywords
primary, bronchial, tracheobronchial, epithelial, culture
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- McCulloughMethodspHBECCulture.docx
Word document version of this method.
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Version History
CURRENT STATUS: Posted
Version 1
Posted 16 Mar, 2021
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Shaun D. McCullough
US Environmental Protection Agency
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6660-346X
Version History
CURRENT STATUS: Posted
Version 1
Posted 16 Mar, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol is intended for culture of primary human tracheobronchial epithelial cells (pHBEC) obtained by brush biopsy during clinical bronchoscopy, or purchased commercially, using Lonza-based medium.
Note: This is a historical protocol. At the time of publication, this protocol has been superseded by a different version in the McCullough lab; however, it is being published to support the transparency and reproducibility of other studies by which it is referenced.
Disclaimer: The information presented here has been reviewed and approved for publication by the US Environmental Protection Agency do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use.
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- McCulloughMethodspHBECCulture.docx
Word document version of this method.
Loading...