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Method Article
Adriana A. S. Tavares
University of Edinburgh
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
18F-sodium fluoride (18F-NaF) is a positron emission tomography (PET) radiotracer widely used in skeletal imaging and has also been proposed as a biomarker of active calcification in atherosclerosis. Like most PET radiotracers, 18F-NaF is typically administered intravenously. However in small animal research intravenous administrations can be challenging, because partial paravenous injection is common due to the small calibre of the superficial tail veins and repeat administrations via tail veins can lead to tissue injury therefore limiting the total number of longitudinal scanning points. This protocol allows to look at the feasibility of using intra-peritoneal route of injection of 8F-NaF to study calcification in mice by looking at the kinetic and uptake profiles of normal soft tissues and bones versus intra-vascular injections.
Keywords
18F-NaF; calcification; intraperitoneal injection; CT; PET imaging
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Posted 22 Apr, 2021
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Biological techniques
Associated Publications
Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
by Zaniah N. Gonzalez-Galofre, Carlos J. Alcaide-Corral, Adriana A. S. Tavares
Scientific Reports (09 March, 2021)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Adriana A. S. Tavares
University of Edinburgh
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 22 Apr, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY 4.0 License. Read Full License
18F-sodium fluoride (18F-NaF) is a positron emission tomography (PET) radiotracer widely used in skeletal imaging and has also been proposed as a biomarker of active calcification in atherosclerosis. Like most PET radiotracers, 18F-NaF is typically administered intravenously. However in small animal research intravenous administrations can be challenging, because partial paravenous injection is common due to the small calibre of the superficial tail veins and repeat administrations via tail veins can lead to tissue injury therefore limiting the total number of longitudinal scanning points. This protocol allows to look at the feasibility of using intra-peritoneal route of injection of 8F-NaF to study calcification in mice by looking at the kinetic and uptake profiles of normal soft tissues and bones versus intra-vascular injections.
Loading...
Associated Publications
Effects of administration route on uptake kinetics of 18F-sodium fluoride positron emission tomography in mice
by Zaniah N. Gonzalez-Galofre, Carlos J. Alcaide-Corral, Adriana A. S. Tavares
Scientific Reports (09 March, 2021)