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Method Article
Michelle Dimon
Google Research
Corresponding Author
B. Scott Ferguson
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Aptamers are single-stranded nucleic acid ligands that bind to target molecules with high affinity and specificity. They are typically discovered by searching large libraries for sequences with desirable binding properties. These libraries, however, are practically constrained to a fraction of the theoretical sequence space. Machine learning provides an opportunity to intelligently navigate this space to identify high-performing aptamers. Here, we present a step-by-step protocol for utilizing particle display to select DNA aptamers for a 25 kDa protein biomarker neutrophil gelatinase-associated lipocalin (NGAL).
Keywords
aptamer selection, aptamer screening, particle display, directed evolution; high-throughput screening
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Posted 30 Apr, 2021
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Subject Areas
Biological techniques
Associated Publications
Machine learning guided aptamer refinement and discovery
by Ali Bashir, Qin Yang, Jinpeng Wang, +14
Nature Communications (22 April, 2021)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Michelle Dimon
Google Research
Corresponding Author
B. Scott Ferguson
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 30 Apr, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Aptamers are single-stranded nucleic acid ligands that bind to target molecules with high affinity and specificity. They are typically discovered by searching large libraries for sequences with desirable binding properties. These libraries, however, are practically constrained to a fraction of the theoretical sequence space. Machine learning provides an opportunity to intelligently navigate this space to identify high-performing aptamers. Here, we present a step-by-step protocol for utilizing particle display to select DNA aptamers for a 25 kDa protein biomarker neutrophil gelatinase-associated lipocalin (NGAL).
Loading...
Associated Publications
Machine learning guided aptamer refinement and discovery
by Ali Bashir, Qin Yang, Jinpeng Wang, +14
Nature Communications (22 April, 2021)