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Method Article
Jun Wu
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The recent development of stem-cell-based embryo models open new avenues for studying early development in vitro, including humans1. Here we report a protocol for the generation of human blastocyst-like structures from naïve human pluripotent stem cells (hPSCs). These structures, termed human blastoids, provide an accessible, scalable, versatile, and manipulable 3D model to study early human development, understand early pregnancy loss, and gain insights into early developmental defects.
Keywords
Human pluripotent stem cells, Naive pluripotent stem cells, stem cell model, blastocyst-like structure
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Jun Wu
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 18 Mar, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Stem cells
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The recent development of stem-cell-based embryo models open new avenues for studying early development in vitro, including humans1. Here we report a protocol for the generation of human blastocyst-like structures from naïve human pluripotent stem cells (hPSCs). These structures, termed human blastoids, provide an accessible, scalable, versatile, and manipulable 3D model to study early human development, understand early pregnancy loss, and gain insights into early developmental defects.
Figure 1
Figure 2
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