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Method Article
Jose M. Polo
Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia, Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Clayton, Victoria, Australia, Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
In the human blastocyst, as development progresses, cells of the epiblast (EPI) lineage will develop into the embryo proper and amnion, whereas cells of the trophectoderm (TE) and primitive endoderm (PE) will give rise to the placenta and yolk sac, respectively1. The study of early human development relies on the use of blastocysts donated to research or cell culture systems such as pluripotent and trophoblast stem cells. Altogether, these have been seminal in shedding light on many key differentiation processes. However, simple culture systems lack the necessary complexity to adequately model the spatio-temporal/cellular and molecular dynamics occurring during the early phases of embryonic development. Currently, an in vitro model of the human blastocyst is not available. Here, we describe a protocol for the generation of an in vitro integrated model of the human blastocyst obtained by reprogramming fibroblasts, termed iBlastoids.
Keywords
iBlastoids, Reprogramming
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Associated Publications
Modelling human blastocysts by reprogramming fibroblasts into iBlastoids
by Xiaodong Liu, Jia Ping Tan, Jan Schröder, +14
Nature (17 March, 2021)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Jose M. Polo
Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia, Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Clayton, Victoria, Australia, Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 25 Mar, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Cell Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
In the human blastocyst, as development progresses, cells of the epiblast (EPI) lineage will develop into the embryo proper and amnion, whereas cells of the trophectoderm (TE) and primitive endoderm (PE) will give rise to the placenta and yolk sac, respectively1. The study of early human development relies on the use of blastocysts donated to research or cell culture systems such as pluripotent and trophoblast stem cells. Altogether, these have been seminal in shedding light on many key differentiation processes. However, simple culture systems lack the necessary complexity to adequately model the spatio-temporal/cellular and molecular dynamics occurring during the early phases of embryonic development. Currently, an in vitro model of the human blastocyst is not available. Here, we describe a protocol for the generation of an in vitro integrated model of the human blastocyst obtained by reprogramming fibroblasts, termed iBlastoids.
Loading...
Associated Publications
Modelling human blastocysts by reprogramming fibroblasts into iBlastoids
by Xiaodong Liu, Jia Ping Tan, Jan Schröder, +14
Nature (17 March, 2021)