Chemicals
Reagent Manufacturer Catalog #
WarmStart® Colorimetric LAMP 2X Master Mix New England Biolabs M1800L
ATP (100mM) Thermo Fisher Scientific R0441
Protector RNase Inhibitor (40U/µl) Roche 3335399001
Thermostable Inorganic Pyrophosphatase (2U/µl) New England Biolabs M0296L
Tte UvrD Helicase (20ng/ µl) New England Biolabs M1202S
SYTO-9 (5mM) Thermo Fisher Scientific S34854
SYBR green I (10000x) Thermo Fisher Scientific S7563
Dynabeads™ MyOne™ Streptavidin C1 Thermo Fisher Scientific 65001
Tris-HCL (1M, pH 7.5) Jena Bioscience BU-125L
LiCl Alfa Aesar 10515.3
Lithium dodecyl sulfate VWR Chemicals 782-25G
Dithiothreitol Molekula Group 19733320
EDTA (0.5M, pH 8.0) VWR Chemicals E522-100ML
Tween-20 Thermo Fisher Scientific 85113
Sodium hydroxyde (1M) Sigma Aldrich S2770
Twist synthetic SARS-CoV-2 RNA control 2 Twist Bioscience 102024
Oligonucleotides (Sigma Aldrich, desalted)
ID Sequence (5‘ -> 3‘)
CV1 (F3) TCCAGATGAGGATGAAGAAGA
CV2 (B3) AGTCTGAACAACTGGTGTAAG
CV3 (FIP) AGAGCAGCAGAAGTGGCACAGGTGATTGTGAAGAAGAAGAG
CV4 (BIP) TCAACCTGAAGAAGAGCAAGAACTGATTGTCCTCACTGCC
CV5 (LF) CTCATATTGAGTTGATGGCTCA
CV6 (LB) ACAAACTGTTGGTCAACAAGAC
CV15 (F3) TGGCTACTACCGAAGAGCT
CV16 (B3) TGCAGCATTGTTAGCAGGAT
CV17 (FIP) TCTGGCCCAGTTCCTAGGTAGTCCAGACGAATTCGTGGTGG
CV18 (BIP) AGACGGCATCATATGGGTTGCACGGGTGCCAATGTGATCT
CV19 (LF) GGACTGAGATCTTTCATTTTACCGT
CV20 (LB) ACTGAGGGAGCCTTGAATACA
CV2_btn (capture oligonucleotide) /BtnTg/TTAAATAACCACTAAAACTATTCACTTCAATAGTCTGAACAACTGGTGTAAG
CV16_btn (capture oligonucleotide) /BtnTg/AATGTTGTTCCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGCAGGAT
Recipe Low Salt buffer
Reagent Amount Final concentration
Tris HCl (1M, pH 7.5) 4ml 20mM
LiCl 1.694g 200mM
EDTA (0.5M, pH 8.0) 0.4ml 1mM
Water to 200ml
Recipe Elution buffer
Reagent Amount Final concentration
Tris HCl (1M, pH 7.5) 4ml 20mM
EDTA (0.5M, pH 8.0) 0.4ml 1mM
Water to 200ml
Recipe Wash buffer
Reagent Amount Final concentration
Tris HCl (1M, pH 7.5) 4ml 20mM
LiCl 4.235g 500mM
EDTA (0.5M pH 8.0) 0.4ml 1mM
Water to 200ml
Recipe Lysis Binding Buffer (2x)
Reagent Amount Final concentration
Tris HCl (1M, pH 7.5) 40ml 200mM
LiCl 8.47g 500mM
EDTA (0.5M pH 8.0) 0.8ml 2mM
LiDS 2g 1% (w/V)
DTT 0.3085g 10mM
Water to 200ml
Recipe alkaline melt solution
Reagent Amount Final concentration
Tween 20 1µl 0.1% (v/v)
NaOH (1M) 125µl 125mM
Water to 1000µM
Recipe primer mix (10x)
Primer V/µl Final concentration (10x)
F3 (100µM) 8 2µM
B3 (100µM) 8 2µM
FIP (100µM) 64 16µM
BIP (100µM) 64 16µM
LF (100µM) 16 4µM
LB (100µM) 16 4µM
Water to 400µl
Recipe LAMP master mix
Reagent V/µl Final concentration
WarmStart LAMP MM (2x) 15 1x
ATP (100mM) 0.3 1mM
RNase In (40U/µl, Roche) 0.3 0.4U/µl
Thermostable PPase (2 U/µl) 0.75 0.05U/µl
Primer mix (10x) 3 1x Water 0.2 4
Tte UvrD helicase (20ng/µl) 0.15 1ng/µl
SYTO-9 (100µM) 0.3 1µM