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Method Article
Ingrid Herr
Section Surgical Research, Molecular OncoSurgery, Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2389-6300
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Tumor xenotransplantation to the chorioallantois membrane (CAM) of fertilized chicken eggs is excellently suited to short-time analysis of xenograft growth and can reduce mouse experiments. However, the limiting factor with egg xenograft experiments is the high price and limited availability of Thermanox plastic cell culture coverslips, which are often sold out and unavailable for weeks. According to the regular protocol, rings are punched from Thermanox plastic cell culture coverslips 13 mm ø and sticked to the CAM. The xenografts are then sown into these rings in order to prevent that they slide around on the CAM. Thermanox rings are well tolerated and do not interfere with the development of chicken embryos. Here we present a method for preparation of comparable, cheaper and always available alternative rings. We evaluated self-made rings from agar and PVC tube and compared their suitability to Thermanox rings. We found that rings of agar and PVC tube achieved the same xenograft sizes and had no side effects on chick embryonal growth, and therefore seem to be as as good as Thermanox rings.
Keywords
CAM assay, fertilized chicken eggs, CAM xenotransplantation, tumor xenografts, mammalian replacement method, thermanox coverslips alternative
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Associated Publications
MicroRNA in vivo delivery to human pancreas tumor xenografts on chicken eggs
by Ingrid Herr, Clifford C. Nwaeburu, Ewa Aleksandrowicz, +3
Protocol Exchange (08 November, 2017)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Ingrid Herr
Section Surgical Research, Molecular OncoSurgery, Department of General, Visceral and Transplantation Surgery, University of Heidelberg, Heidelberg, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2389-6300
Version History
CURRENT STATUS: Posted
Version 1
Posted 19 Jan, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Tumor xenotransplantation to the chorioallantois membrane (CAM) of fertilized chicken eggs is excellently suited to short-time analysis of xenograft growth and can reduce mouse experiments. However, the limiting factor with egg xenograft experiments is the high price and limited availability of Thermanox plastic cell culture coverslips, which are often sold out and unavailable for weeks. According to the regular protocol, rings are punched from Thermanox plastic cell culture coverslips 13 mm ø and sticked to the CAM. The xenografts are then sown into these rings in order to prevent that they slide around on the CAM. Thermanox rings are well tolerated and do not interfere with the development of chicken embryos. Here we present a method for preparation of comparable, cheaper and always available alternative rings. We evaluated self-made rings from agar and PVC tube and compared their suitability to Thermanox rings. We found that rings of agar and PVC tube achieved the same xenograft sizes and had no side effects on chick embryonal growth, and therefore seem to be as as good as Thermanox rings.
Figure 1
Figure 2
Figure 3
Loading...
Associated Publications
MicroRNA in vivo delivery to human pancreas tumor xenografts on chicken eggs
by Ingrid Herr, Clifford C. Nwaeburu, Ewa Aleksandrowicz, +3
Protocol Exchange (08 November, 2017)