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Method Article
Bing Ren
Ludwig Institute for Cancer Research, 3Center for Epigenomics, Department of Cellular and Molecular Medicine, Institute of Genomic Medicine, Moores Cancer Center, University of California San Diego, School of Medicine, La Jolla, California, USA
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5435-1127
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We describe here Paired-Tag, a high-throughput multi-omics method for joint profiling of histone modifications and gene expressions in single cells. The assay is based on a combinatorial barcoding indexing strategy that does not require special instruments. It can be performed with nuclei extracted from cultured cells or frozen tissues, in standard molecular biology laboratories.
Keywords
Paired-Tag, Single-cell multi-omics, Histone modifications, Gene expression
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Bing Ren
Ludwig Institute for Cancer Research, 3Center for Epigenomics, Department of Cellular and Molecular Medicine, Institute of Genomic Medicine, Moores Cancer Center, University of California San Diego, School of Medicine, La Jolla, California, USA
Corresponding Author
ORCiD: https://orcid.org/0000-0002-5435-1127
Version History
CURRENT STATUS: Posted
The most recent version of this article is available here.
Community comments: 15
Version 1
Posted 15 Feb, 2021
Community comments: 4
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The most recent version of this article is available here.
We describe here Paired-Tag, a high-throughput multi-omics method for joint profiling of histone modifications and gene expressions in single cells. The assay is based on a combinatorial barcoding indexing strategy that does not require special instruments. It can be performed with nuclei extracted from cultured cells or frozen tissues, in standard molecular biology laboratories.
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