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Method Article
Kristiyan Kanev
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2705-9307
Dietmar Zehn
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
tSCBR-seq is a T-cell tailored plate-based single-cell RNA sequencing protocol with superior mRNA capturing efficacy and dynamic range of gene detection. This was achieved through a series of chemical modifications of the original SCBR-seq protocol published by Soumilon and colleagues [1]. The performance of the protocol was also demonstrated in the article “Proliferation-competent Tcf1+ CD8 T cells in dysfunctional populations are CD4 T cell help independent” [2].
Keywords
primary T cells, single-cell RNA sequencing
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- tSCRBBarcodedOligodTPrimerPlatev3sequences.xls
tSCRB Barcoded Oligo-dT Primer Plate v3
- Table3.png
Table 3
- Table1.png
Table 1
- Table2.png
Table 2
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Associated Publications
Method Article
Kristiyan Kanev
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2705-9307
Dietmar Zehn
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 08 Jan, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
tSCBR-seq is a T-cell tailored plate-based single-cell RNA sequencing protocol with superior mRNA capturing efficacy and dynamic range of gene detection. This was achieved through a series of chemical modifications of the original SCBR-seq protocol published by Soumilon and colleagues [1]. The performance of the protocol was also demonstrated in the article “Proliferation-competent Tcf1+ CD8 T cells in dysfunctional populations are CD4 T cell help independent” [2].
Figure 1
Figure 2
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