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Method Article
T-cell tailored single-cell RNA barcoding and sequencing (tSCRB-seq)
Kristiyan Kanev
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2705-9307
Dietmar Zehn
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
tSCBR-seq is a T-cell tailored plate-based single-cell RNA sequencing protocol with superior mRNA capturing efficacy and dynamic range of gene detection. This was achieved through a series of chemical modifications of the original SCBR-seq protocol published by Soumilon and colleagues [1]. The performance of the protocol was also demonstrated in the article “Proliferation-competent Tcf1+ CD8 T cells in dysfunctional populations are CD4 T cell help independent” [2].
Keywords
primary T cells, single-cell RNA sequencing
Figure 1
Figure 2
Due to technical limitations, Tables 1-3 can be found in the Supplementary Files section
This is a list of supplementary files associated with this preprint. Click to download.
- tSCRBBarcodedOligodTPrimerPlatev3sequences.xls
tSCRB Barcoded Oligo-dT Primer Plate v3
- Table3.png
Table 3
- Table1.png
Table 1
- Table2.png
Table 2
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This is a preprint. It has not completed peer review.
Method Article
T-cell tailored single-cell RNA barcoding and sequencing (tSCRB-seq)
Kristiyan Kanev
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2705-9307
Dietmar Zehn
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 08 Jan, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
tSCBR-seq is a T-cell tailored plate-based single-cell RNA sequencing protocol with superior mRNA capturing efficacy and dynamic range of gene detection. This was achieved through a series of chemical modifications of the original SCBR-seq protocol published by Soumilon and colleagues [1]. The performance of the protocol was also demonstrated in the article “Proliferation-competent Tcf1+ CD8 T cells in dysfunctional populations are CD4 T cell help independent” [2].
Figure 1
Figure 2
Due to technical limitations, Tables 1-3 can be found in the Supplementary Files section