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Method Article
Kristiyan Kanev
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2705-9307
Dietmar Zehn
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
tSCBR-seq is a T-cell tailored plate-based single-cell RNA sequencing protocol with superior mRNA capturing efficacy and dynamic range of gene detection. This was achieved through a series of chemical modifications of the original SCBR-seq protocol published by Soumilon and colleagues [1]. The performance of the protocol was also demonstrated in the article “Proliferation-competent Tcf1+ CD8 T cells in dysfunctional populations are CD4 T cell help independent” [2].
Keywords
primary T cells, single-cell RNA sequencing
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- tSCRBBarcodedOligodTPrimerPlatev3sequences.xls
tSCRB Barcoded Oligo-dT Primer Plate v3
- Table3.png
Table 3
- Table1.png
Table 1
- Table2.png
Table 2
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Associated Publications
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Kristiyan Kanev
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0002-2705-9307
Dietmar Zehn
Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 08 Jan, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
tSCBR-seq is a T-cell tailored plate-based single-cell RNA sequencing protocol with superior mRNA capturing efficacy and dynamic range of gene detection. This was achieved through a series of chemical modifications of the original SCBR-seq protocol published by Soumilon and colleagues [1]. The performance of the protocol was also demonstrated in the article “Proliferation-competent Tcf1+ CD8 T cells in dysfunctional populations are CD4 T cell help independent” [2].
Figure 1
Figure 2
This is a list of supplementary files associated with this preprint. Click to download.
- tSCRBBarcodedOligodTPrimerPlatev3sequences.xls
tSCRB Barcoded Oligo-dT Primer Plate v3
- Table3.png
Table 3
- Table1.png
Table 1
- Table2.png
Table 2
Loading...