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Method Article
Ampule-sealed Acidolysis for Monosaccharide Composition Analysis of Serum or Plasma Samples
Lijuan Zhang
Corresponding Author
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This protocol describes the procedures where an ampule-sealed acidolysis is used for releasing glycan monosaccharides from serum or plasma samples. The monosaccharide composition analysis is subsequently obtained by a traditional HPLC method that separates and quantifies all 1-phenyl-3-methyl-5-pyrazolone (PMP)-labeled monosaccharides in 10 μl serum in 55 minutes. The described workflow takes approximately 2 days, up to 26 serum samples can be analyzed with one HPLC instrument per day. Each step of the experimental procedures has been optimized with repeated tests of monosaccharide standards and serum samples.
Keywords
serum, plasma, glycan, ampule-sealed acidolysis, 1-phenyl-3-methyl-5-pyrazolone (PMP), derivatization, HPLC, monosaccharide compositions
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This is a preprint. It has not completed peer review.
Method Article
Ampule-sealed Acidolysis for Monosaccharide Composition Analysis of Serum or Plasma Samples
Lijuan Zhang
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 23 Dec, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Chemical biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes the procedures where an ampule-sealed acidolysis is used for releasing glycan monosaccharides from serum or plasma samples. The monosaccharide composition analysis is subsequently obtained by a traditional HPLC method that separates and quantifies all 1-phenyl-3-methyl-5-pyrazolone (PMP)-labeled monosaccharides in 10 μl serum in 55 minutes. The described workflow takes approximately 2 days, up to 26 serum samples can be analyzed with one HPLC instrument per day. Each step of the experimental procedures has been optimized with repeated tests of monosaccharide standards and serum samples.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5