Ampule-sealed Acidolysis for Monosaccharide Composition Analysis of Serum or Plasma Samples
This protocol describes the procedures where an ampule-sealed acidolysis is used for releasing glycan monosaccharides from serum or plasma samples. The monosaccharide composition analysis is subsequently obtained by a traditional HPLC method that separates and quantifies all 1-phenyl-3-methyl-5-pyrazolone (PMP)-labeled monosaccharides in 10 μl serum in 55 minutes. The described workflow takes approximately 2 days, up to 26 serum samples can be analyzed with one HPLC instrument per day. Each step of the experimental procedures has been optimized with repeated tests of monosaccharide standards and serum samples.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Posted 23 Dec, 2020
Ampule-sealed Acidolysis for Monosaccharide Composition Analysis of Serum or Plasma Samples
Posted 23 Dec, 2020
This protocol describes the procedures where an ampule-sealed acidolysis is used for releasing glycan monosaccharides from serum or plasma samples. The monosaccharide composition analysis is subsequently obtained by a traditional HPLC method that separates and quantifies all 1-phenyl-3-methyl-5-pyrazolone (PMP)-labeled monosaccharides in 10 μl serum in 55 minutes. The described workflow takes approximately 2 days, up to 26 serum samples can be analyzed with one HPLC instrument per day. Each step of the experimental procedures has been optimized with repeated tests of monosaccharide standards and serum samples.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
© Research Square 2021