A protocol for exosome detection via the ultrafast-isolation system (EXODUS)

doi:10.21203/rs.3.pex-1263/v1

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Yuchao Chen, Qingfu Zhu, Luke P. Lee , Fei Liu

Yuchao Chen

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Qingfu Zhu

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Luke P. Lee

Department of Medicine, Division of Engineering in Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, United States

Corresponding Author

ORCiD: https://orcid.org/0000-0001-9663-3109

Fei Liu

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Corresponding Author

ORCiD: https://orcid.org/0000-0001-5259-5753

DOI:

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Here we present a protocol for isolation and purification of exosomes using exosome detection via the ultrafast-isolation system (EXODUS). This protocol is associated with our Nature Methods paper: Exosome Detection via the Ultrafast-isolation System: EXODUS.

Keywords

EXODUS, isolation, exosome, extracellular vesicle

Version History

CURRENT STATUS: Posted

Version 1

Posted 11 Jan, 2021

Community comments: 1

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Biological techniques

Biotechnology

Analytical chemistry

Molecular biology

Associated Publications

10.1038/s41592-020-01034-x

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Yuchao Chen, Qingfu Zhu, Luke P. Lee , Fei Liu

Yuchao Chen

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Qingfu Zhu

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Luke P. Lee

Department of Medicine, Division of Engineering in Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, United States

Corresponding Author

ORCiD: https://orcid.org/0000-0001-9663-3109

Fei Liu

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Corresponding Author

ORCiD: https://orcid.org/0000-0001-5259-5753

Version History

CURRENT STATUS: Posted

Version 1

Posted 11 Jan, 2021

Community comments: 1

MetricsComments: 0HTML Views: ...

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Yuchao Chen, Qingfu Zhu, Luke P. Lee , Fei Liu

Yuchao Chen

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Qingfu Zhu

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Luke P. Lee

Department of Medicine, Division of Engineering in Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, United States

Corresponding Author

ORCiD: https://orcid.org/0000-0001-9663-3109

Fei Liu

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Corresponding Author

ORCiD: https://orcid.org/0000-0001-5259-5753

DOI:

10.21203/rs.3.pex-1263/v1

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

EXODUS, isolation, exosome, extracellular vesicle

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Time Taken

Anticipated Results

Acknowledgements

Comments

Version History

CURRENT STATUS: Posted

Version 1

Community comments: 1

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Biological techniques

Biotechnology

Analytical chemistry

Molecular biology

Associated Publications

10.1038/s41592-020-01034-x

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Yuchao Chen, Qingfu Zhu, Luke P. Lee , Fei Liu

Yuchao Chen

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Qingfu Zhu

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Luke P. Lee

Department of Medicine, Division of Engineering in Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, United States

Corresponding Author

ORCiD: https://orcid.org/0000-0001-9663-3109

Fei Liu

School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China

Corresponding Author

ORCiD: https://orcid.org/0000-0001-5259-5753

Badges

Peer Review Timeline

Version History

CURRENT STATUS: Posted

Version 1

Community comments: 1

Metrics

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Subject Areas

Biological techniques

Biotechnology

Analytical chemistry

Molecular biology

DOI & PDF