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Method Article
Christian Frøkjær-Jensen
King Abdullah University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3178-0906
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Transgenes are particularly prone to epigenetic silencing in the C. elegans germline. Here, we describe a protocol to insert introns containing a class of non-coding DNA named Periodic An/Tn Clusters (PATCs) into synthetic transgenes. PATCs can protect transgenes from position-dependent silencing (Position Effect Variegation, PEV) and from silencing in simple extra-chromosomal arrays. Using a set of simple design rules, it is possible to routinely insert up to three PATC-rich introns into a synthetic transgene in a single reaction.
Keywords
C. elegans, epigenetic silencing, non-coding DNA, insulator
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Associated Publications
Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans
by Mohammed D. Aljohani, Sonia El Mouridi, Monika Priyadarshini, +2
Nature Communications (09 December, 2020)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Christian Frøkjær-Jensen
King Abdullah University of Science and Technology
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3178-0906
Version History
CURRENT STATUS: Posted
Version 1
Posted 15 Feb, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Genetics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Transgenes are particularly prone to epigenetic silencing in the C. elegans germline. Here, we describe a protocol to insert introns containing a class of non-coding DNA named Periodic An/Tn Clusters (PATCs) into synthetic transgenes. PATCs can protect transgenes from position-dependent silencing (Position Effect Variegation, PEV) and from silencing in simple extra-chromosomal arrays. Using a set of simple design rules, it is possible to routinely insert up to three PATC-rich introns into a synthetic transgene in a single reaction.
Loading...
Associated Publications
Engineering rules that minimize germline silencing of transgenes in simple extrachromosomal arrays in C. elegans
by Mohammed D. Aljohani, Sonia El Mouridi, Monika Priyadarshini, +2
Nature Communications (09 December, 2020)