This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Expansion microscopy of cellular and bacterial membranes by functionalized ceramides
Markus Sauer
University of Wuerzburg
Corresponding Author
Thomas Rudel
Vera Kozjak-Pavlovic
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Due to the lack of primary amino groups, lipids cannot be fixed by formaldehyde, glutaraldehyde and other chemical fixatives nor expanded using expansion microscopy (ExM). We present a protocol that uses unnatural short-chain azide- and amino-functionalized ceramides, which upon incorporation into cellular and bacterial membranes can be labeled by click chemistry and linked into hydrogels, followed by 4x to 10x expansion.
Keywords
super-resolution imaging, expansion microscopy, membranes
Figure 1
Figure 1
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 02 Dec, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biophysics
Associated Publications
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Expansion microscopy of cellular and bacterial membranes by functionalized ceramides
Markus Sauer
University of Wuerzburg
Corresponding Author
Thomas Rudel
Vera Kozjak-Pavlovic
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 02 Dec, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biophysics
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Due to the lack of primary amino groups, lipids cannot be fixed by formaldehyde, glutaraldehyde and other chemical fixatives nor expanded using expansion microscopy (ExM). We present a protocol that uses unnatural short-chain azide- and amino-functionalized ceramides, which upon incorporation into cellular and bacterial membranes can be labeled by click chemistry and linked into hydrogels, followed by 4x to 10x expansion.
Figure 1
Figure 1