Mouse fetal liver erythroid population separation using FACS.

doi:10.21203/rs.3.pex-1246/v1

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Method Article

Robert A. Beagrie, Damien J. Downes

Robert A. Beagrie

Oxford University

Corresponding Author

ORCiD: https://orcid.org/0000-0003-1076-5595

Damien J. Downes

Oxford University

ORCiD: https://orcid.org/0000-0002-5034-0869

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Through-out erythropoiesis cells undergo several morphological and regulatory changes. In mouse, these changes can be characterized through isolation of specific intermediate populations based on the surface levels of Ter119 (Glycophorin A-associated protein/Ly-76) and CD71 (Transferrin receptor 1)^1-2. This method takes roughly one day and can be used to generate sufficient cells for most high-throughput sequencing techniques with low input adaptations.

Keywords

Erythropoiesis, FACS, erythroid

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High-resolution targeted 3C interrogation of cis-regulatory element organization at genome-wide scale
by Damien J. Downes, Robert A. Beagrie, Matthew E. Gosden, +16
Nature Communications (22 January, 2021)

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Method Article

Robert A. Beagrie, Damien J. Downes

Robert A. Beagrie

Oxford University

Corresponding Author

ORCiD: https://orcid.org/0000-0003-1076-5595

Damien J. Downes

Oxford University

ORCiD: https://orcid.org/0000-0002-5034-0869

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CURRENT STATUS: Posted

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Posted 28 Jan, 2021

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Biological techniques

DOI:

10.21203/rs.3.pex-1246/v1

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The full text of this article is available to read as a PDF.

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Associated Publications

High-resolution targeted 3C interrogation of cis-regulatory element organization at genome-wide scale
by Damien J. Downes, Robert A. Beagrie, Matthew E. Gosden, +16
Nature Communications (22 January, 2021)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Robert A. Beagrie, Damien J. Downes

Robert A. Beagrie

Oxford University

Corresponding Author

ORCiD: https://orcid.org/0000-0003-1076-5595

Damien J. Downes

Oxford University

ORCiD: https://orcid.org/0000-0002-5034-0869

DOI:

10.21203/rs.3.pex-1246/v1

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

Erythropoiesis, FACS, erythroid

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High-resolution targeted 3C interrogation of cis-regulatory element organization at genome-wide scale

by Damien J. Downes, Robert A. Beagrie, Matthew E. Gosden, +16

Nature Communications (22 January, 2021)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Robert A. Beagrie, Damien J. Downes

Robert A. Beagrie

Oxford University

Corresponding Author

ORCiD: https://orcid.org/0000-0003-1076-5595

Damien J. Downes

Oxford University

ORCiD: https://orcid.org/0000-0002-5034-0869

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DOI:

10.21203/rs.3.pex-1246/v1

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Associated Publications

High-resolution targeted 3C interrogation of cis-regulatory element organization at genome-wide scale