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4sU-seq allows for measuring or nascent transcription which can reflect dynamic changes more accurately than traditional RNA-seq. Incorporation of the nucleoside analog 4-thiouridine into RNA allows the separation of nascent transcripts though reversible coupling to biotin and streptavdin bead pull-down. The protocol takes roughly two days to generate a sequencing ready library.
Keywords
Gene expression, gene regulation, transcription
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Associated Publications
High-resolution targeted 3C interrogation of cis-regulatory element organization at genome-wide scale
by Damien J. Downes, Robert A. Beagrie, Matthew E. Gosden, +16
Nature Communications (22 January, 2021)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Version History
CURRENT STATUS: Posted
Version 1
Posted 28 Jan, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY 4.0 License. Read Full License
4sU-seq allows for measuring or nascent transcription which can reflect dynamic changes more accurately than traditional RNA-seq. Incorporation of the nucleoside analog 4-thiouridine into RNA allows the separation of nascent transcripts though reversible coupling to biotin and streptavdin bead pull-down. The protocol takes roughly two days to generate a sequencing ready library.
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Associated Publications
High-resolution targeted 3C interrogation of cis-regulatory element organization at genome-wide scale
by Damien J. Downes, Robert A. Beagrie, Matthew E. Gosden, +16
Nature Communications (22 January, 2021)