**Sample Preparation**

1.- Homogenize 50 mg of dry foliar tissue using an ice-cold mortar and pestle, and 2 mL of cold methanol (95%).

2.- Place the sample in a 2 mL microtube and vortex for 10 seconds.

3.- Incubate at room temperature for 48 h in the dark.

4.- Vortex the sample for 10 seconds and centrifuge it at 5000 rpm for 4 minutes.

**Total Phenols Analysis **

5.- Transfer 40 mL of supernatant to a new microtube and add 960 μL of methanol (95%).

6.- Vortex for 10 seconds and transfer 100 mL to a new microtube for Folin-Ciocalteu analysis.

**Folin-Ciocalteu Colorimetric Assay**

7.- Add 200 μL of F-C 10% and vortex for 10 seconds.

8.- Cover and incubate the sample for 30 minutes at ambient temperature.

9.- Add 800 μL of Na2CO3 (700 mM).

10.- Cover and incubate for 2 hours at ambient temperature.

11.- Centrifuge the sample at 5000 rpm for 4 minutes.

12.- Transfer 300 μL to a well in a microplate and read absorbance at 735nm.

**Total Tannins Analysis**

13.- Transfer the 900 μL of supernatant from step 5 to a new microtube containing 20 mg of PVP.

14.- Vortex for 10 seconds and incubate in freezer (-4°C) for 30 minutes.

15.- Prepare a blank microtube with 1 mL of methanol (95%) and 20 mg of PVP.

16.- Centrifuge at 5000 rpm for 10 minutes. Keep cold.

17.- Transfer 100 μL to a new microtube and proceed with the Folin-Ciocalteu analysis (step 7).

**Total Flavonoids Analysis**

18.- Transfer 20 μL of supernatant (step 4) to a new microtube and add 80 μL of dd H2O.

19.- Add 30 μL of NaNO2 (5%) and wait for 5 minutes.

20.- Add 30 μL of AlCl3 (10%) and wait for 1 minute.

21.- Add 200 μL of NaOH (1M).

22.- Add 640 μL of dd H2O.

23.- Vortex for 10 seconds and transfer 300 μL to read absorbance at 510 nm.

**Total Concentrations Calculations**

24.- Calculate a standard curve from the blank-corrected at 765 nm of the gallic acid standards (Fig. 1). Calculate total phenols and total tannins concentrations as gallic acid equivalents using the regression equation of gallic acid standards.

25.- Calculate a standard curve from the blank-corrected at 510 nm of the (+)-catechin standards (Fig. 2). Calculate total flavonoids concentrations as catechin equivalents using the regression equation of catechin standards.