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Method Article
Xiang-Hong Ou
Corresponding Author
Zhen-Bo Wang
Corresponding Author
Shao-Rong Gao
Corresponding Author
Qing-Yuan Sun
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol presents ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) assay to generate high quality and complexity genome-wide histone mark profiles from rare oocytes andembryos populations. The procedure of ULI-NChIP-seq assay typically consists of five parts including Binding antibodies to magnatic beads, Chromatin shearing and nuclear membrane solubilization, Magnetic immunoprecipitation, Washes and DNA isolation. Sample preparation involves to remove the zona Pellucida of oocyte and polar body to avoid the genomic contamination of polar bodies.
Keywords
ULI-NChIP, oocyte, histone modification
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Associated Publications
Distinct features of H3K4me3 and H3K27me3 chromatin domains in pre-implantation embryos
by Xiaoyu Liu, Chenfei Wang, Wenqiang Liu, +10
Nature (13 September, 2016)
PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome
by Tie-Gang Meng, Qian Zhou, Xue-Shan Ma, +13
Nature Communications (11 December, 2020)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Xiang-Hong Ou
Corresponding Author
Zhen-Bo Wang
Corresponding Author
Shao-Rong Gao
Corresponding Author
Qing-Yuan Sun
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 26 May, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol presents ULI-NChIP-seq (ultra-low-input micrococcal nuclease-based native ChIP-seq) assay to generate high quality and complexity genome-wide histone mark profiles from rare oocytes andembryos populations. The procedure of ULI-NChIP-seq assay typically consists of five parts including Binding antibodies to magnatic beads, Chromatin shearing and nuclear membrane solubilization, Magnetic immunoprecipitation, Washes and DNA isolation. Sample preparation involves to remove the zona Pellucida of oocyte and polar body to avoid the genomic contamination of polar bodies.
Figure 1
Figure 2
Loading...
Associated Publications
Distinct features of H3K4me3 and H3K27me3 chromatin domains in pre-implantation embryos
by Xiaoyu Liu, Chenfei Wang, Wenqiang Liu, +10
Nature (13 September, 2016)
PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome
by Tie-Gang Meng, Qian Zhou, Xue-Shan Ma, +13
Nature Communications (11 December, 2020)