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Method Article
Bichun Li
Yangzhou University
Corresponding Author
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The chicken primordial germ cell (PGCs) has the unique characteristic of settling in gonad through blood migration, which was the only way to realize the recovery of bird species. However, the PGCs obtained from a single embryo was far from enough to meet the practical application, while somatic cells can be obtained in large quantities. Therefore, the problem of insufficient PGCs can be solved by the induction of somatic cells into PGCs. Here, we successfully transdifferentiate somatic cells into PGCs, which can be transplanted to the recipient to produce offspring. In detail, The CEF of Black-Feathered Langshan Chicken was reprogrammed into iPS by reprogramming factors Oct4, Sox2, Nanog and Lin28, then was induced into PGCs by BMP4/BMP8b/EGF system. The induced PGCs has similar biological characteristics to the primary PGCs, which was transplanted into White Plymouth Rock Chicken, which self-crossed to produce clone-like offspring. It was the the first time to demonstrate the feasibility of avian cloning from somatic cells.
Keywords
Chicken Primordial Germ Cells（cPGCs）；Trnasgenetic Chciken；Reprogramming
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Associated Publications
Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells
by Ruifeng Zhao, Qisheng Zuo, Xia Yuan, +20
Nature Communications (20 May, 2021)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Bichun Li
Yangzhou University
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 26 May, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The chicken primordial germ cell (PGCs) has the unique characteristic of settling in gonad through blood migration, which was the only way to realize the recovery of bird species. However, the PGCs obtained from a single embryo was far from enough to meet the practical application, while somatic cells can be obtained in large quantities. Therefore, the problem of insufficient PGCs can be solved by the induction of somatic cells into PGCs. Here, we successfully transdifferentiate somatic cells into PGCs, which can be transplanted to the recipient to produce offspring. In detail, The CEF of Black-Feathered Langshan Chicken was reprogrammed into iPS by reprogramming factors Oct4, Sox2, Nanog and Lin28, then was induced into PGCs by BMP4/BMP8b/EGF system. The induced PGCs has similar biological characteristics to the primary PGCs, which was transplanted into White Plymouth Rock Chicken, which self-crossed to produce clone-like offspring. It was the the first time to demonstrate the feasibility of avian cloning from somatic cells.
Loading...
Associated Publications
Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells
by Ruifeng Zhao, Qisheng Zuo, Xia Yuan, +20
Nature Communications (20 May, 2021)