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Method Article
Xing Chen
College of Chemistry and Molecular Engineering, Peking University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3058-7370
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We describe the development of click-expansion microscopy (Click-ExM), which integrates click-labeling into ExM to enable a“one-stop-shop” method for nanoscale imaging of various types of biomolecules. Using 18 clickable labels for click-ExM imaging of DNA, RNA, proteins, lipids, glycans and small molecules, we demonstrate its universality, compatibility with signal-amplification techniques, and broad applications in cellular and tissue imaging. The click-labeling and ExM steps could be finished within two days. This step-by-step protocol is related to the publication “Click-ExM enables expansion microscopy for all biomolecules” in Nature Methods.
Keywords
Expansion microscopy, Super-resolution, Click chemistry, Metabolic labeling, Chemical biology
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Xing Chen
College of Chemistry and Molecular Engineering, Peking University
Corresponding Author
ORCiD: https://orcid.org/0000-0002-3058-7370
Version History
CURRENT STATUS: Posted
Version 1
Posted 09 Dec, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
We describe the development of click-expansion microscopy (Click-ExM), which integrates click-labeling into ExM to enable a“one-stop-shop” method for nanoscale imaging of various types of biomolecules. Using 18 clickable labels for click-ExM imaging of DNA, RNA, proteins, lipids, glycans and small molecules, we demonstrate its universality, compatibility with signal-amplification techniques, and broad applications in cellular and tissue imaging. The click-labeling and ExM steps could be finished within two days. This step-by-step protocol is related to the publication “Click-ExM enables expansion microscopy for all biomolecules” in Nature Methods.
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