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Method Article
Creation of bladder assembloids by reconstituting tissue stem cell/tumour cell-derived organoids with multiple stromal components
Kunyoo Shin
Corresponding Author
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Current organoid models are limited by their inability to mimic mature organ architecture and associated tissue microenvironments1,2. Here, we create multi-layered normal bladder assembloids and tumour assembloids by reconstituting tissue stem cell/tumour cell-derived organoids with multiple stromal components to represent an organized tissue architecture and functionality of adult bladder, and to mimic in vivo pathophysiological features of patient-derived urothelial carcinoma, respectively. Our assembloid model will facilitate development of an innovative model system for studying tissue regeneration and tumourigenesis at molecular and cellular levels, and also provide a unique experimental tool for establishing novel personalized therapeutic options, such as new drug screening strategies that are customized for different stages and types of cancer in individual patients.
Keywords
Bladder organoid, bladder assembloid, bladder tumour organoid, tissue stroma, tumour microenvironment, bladder cancer, tumour plasticity
This is a list of supplementary files associated with this preprint. Click to download.
- MediumPreparation.pdf
Supplementary Information 1. Assembloid culture medium
- MediumPreparation.pdf
Supplementary Information 1. Assembloid culture medium
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This is a preprint. It has not completed peer review.
Method Article
Creation of bladder assembloids by reconstituting tissue stem cell/tumour cell-derived organoids with multiple stromal components
Kunyoo Shin
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 16 Dec, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Current organoid models are limited by their inability to mimic mature organ architecture and associated tissue microenvironments1,2. Here, we create multi-layered normal bladder assembloids and tumour assembloids by reconstituting tissue stem cell/tumour cell-derived organoids with multiple stromal components to represent an organized tissue architecture and functionality of adult bladder, and to mimic in vivo pathophysiological features of patient-derived urothelial carcinoma, respectively. Our assembloid model will facilitate development of an innovative model system for studying tissue regeneration and tumourigenesis at molecular and cellular levels, and also provide a unique experimental tool for establishing novel personalized therapeutic options, such as new drug screening strategies that are customized for different stages and types of cancer in individual patients.