An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Julián Andrés Castillo Vargas
Universidade Federal Rural da Amazônia
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5163-5127
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Short-chain fatty acid profile (SCFAs) in silage provides significant information regarding its fermentative patter in the rumen. Thus, we developed a protocol to extract, identify, and quantify the SCFAs of silages. The proposed method was tested with satisfactory results in corn silages. Despite literature findings suggest that the most common instrumental method for determining SCFAs is the gas chromatography technique, the liquid chromatography technique using both C18 column and ion-exchange columns can be proposed as analytical technique for SCFAs determination as well. The use of the C18 column in HPLC-DAD has a particular limitation because it does not allow efficient separation of butyric and iso-butyric acids. However, considering that the concentration of iso-butyric acid in silages is significantly lower than that of butyric acid, a good estimation of butyric acid can be obtained.
Keywords
fatty acid extraction, feedstuff, HPLC, volatile fatty acid
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 01 Oct, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Julián Andrés Castillo Vargas
Universidade Federal Rural da Amazônia
Corresponding Author
ORCiD: https://orcid.org/0000-0001-5163-5127
Version History
CURRENT STATUS: Posted
Version 1
Posted 01 Oct, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Short-chain fatty acid profile (SCFAs) in silage provides significant information regarding its fermentative patter in the rumen. Thus, we developed a protocol to extract, identify, and quantify the SCFAs of silages. The proposed method was tested with satisfactory results in corn silages. Despite literature findings suggest that the most common instrumental method for determining SCFAs is the gas chromatography technique, the liquid chromatography technique using both C18 column and ion-exchange columns can be proposed as analytical technique for SCFAs determination as well. The use of the C18 column in HPLC-DAD has a particular limitation because it does not allow efficient separation of butyric and iso-butyric acids. However, considering that the concentration of iso-butyric acid in silages is significantly lower than that of butyric acid, a good estimation of butyric acid can be obtained.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Loading...