Isolation of small extracellular vesicles from cell culture supernatant

doi:10.21203/rs.3.pex-1159/v1

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Method Article

Eun-Ju Im, Chan-Hyeong Lee, Moon-Chang Baek

Eun-Ju Im

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Chan-Hyeong Lee

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Moon-Chang Baek

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Corresponding Author

DOI:

10.21203/rs.3.pex-1159/v1

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This protocol describes how to isolate small extracellular vesicles (sEV) from cell culture media using an ultracentrifuge and analyze the number of sEV by nanoparticle tracking analyzer.

Keywords

exosome, extracellular vesicle, cancer, nanoparticle tracking analyzer, ultracentrifuge

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Associated Publications

Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
by Eun-Ju Im, Chan-Hyeong Lee, Pyong-Gon Moon, +18
Nature Communications (27 March, 2019)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Eun-Ju Im, Chan-Hyeong Lee, Moon-Chang Baek

Eun-Ju Im

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Chan-Hyeong Lee

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Moon-Chang Baek

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Corresponding Author

Version History

CURRENT STATUS: Posted

Version 1

Posted 16 Oct, 2020

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Subject Areas

Cell Biology

Biological techniques

DOI:

10.21203/rs.3.pex-1159/v1

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY 4.0 License. Read Full License

This protocol describes how to isolate small extracellular vesicles (sEV) from cell culture media using an ultracentrifuge and analyze the number of sEV by nanoparticle tracking analyzer.

Associated Publications

Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
by Eun-Ju Im, Chan-Hyeong Lee, Pyong-Gon Moon, +18
Nature Communications (27 March, 2019)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Eun-Ju Im, Chan-Hyeong Lee, Moon-Chang Baek

Eun-Ju Im

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Chan-Hyeong Lee

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Moon-Chang Baek

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Corresponding Author

DOI:

10.21203/rs.3.pex-1159/v1

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

exosome, extracellular vesicle, cancer, nanoparticle tracking analyzer, ultracentrifuge

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Acknowledgements

Comments

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CURRENT STATUS: Posted

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Cell Biology

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Associated Publications

Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A

by Eun-Ju Im, Chan-Hyeong Lee, Pyong-Gon Moon, +18

Nature Communications (27 March, 2019)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Eun-Ju Im, Chan-Hyeong Lee, Moon-Chang Baek

Eun-Ju Im

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Chan-Hyeong Lee

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Moon-Chang Baek

Department of Molecular Medicine, CMRI, Exosome Convergence Research Center (ECRC), School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.

Corresponding Author

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DOI & PDF

DOI:

10.21203/rs.3.pex-1159/v1

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License

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Declarations

Abstract

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Acknowledgements