Explore study: Retrospective Cohort study to show the start of SARS-CoV-2 in patients with severe respiratory illness by real-time PCR

doi:10.21203/rs.3.pex-1157/v1

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Method Article

Sara H.A.Agwa, Hesham Elghazaly, Sarah El-Nakeep, Ahmad Moustafa, Manal H El-Sayed, Hala Hafez, Samia abdo, Marwa Matboli, Maha Saad, Shaimaa M.Elsayed, Aya M.Abd Elsamie, Reham M.Darwish, Hoda ezz elarab, Fatma S. E. Ebeid, Sara Makkeyah, Mahmoud S El Meteini

Sara H.A.Agwa

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Hesham Elghazaly

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Sarah El-Nakeep

Department of Internal Medicine, Ain Shams University

Corresponding Author

Ahmad Moustafa

Department of Biology, American University in Cairo, New Cairo, Egypt

Manal H El-Sayed

• Pediatric Hematology Oncology Department, Ain Shams University

Hala Hafez

Clinical pathology Department, Ain Shams University

Samia abdo

Clinical pathology Department, Ain Shams University

Marwa Matboli

Biochemistry and molecular biology Department, Ain Shams University

Maha Saad

Medicinal biochemistry and molecular biology Department, Modern University for Technology and Information

Shaimaa M.Elsayed

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Aya M.Abd Elsamie

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Reham M.Darwish

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Hoda ezz elarab

Clinical pathology Department, Ain Shams University

Fatma S. E. Ebeid

• Pediatric Hematology Oncology Department, Ain Shams University

Sara Makkeyah

Pediatric Hematology Oncology Department, Ain Shams University

Mahmoud S El Meteini

Department of General Surgery, Ain Shams University

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Aim: to know the start of COVID-19 in Ain shams University hospitals (El-Demerdash) in Severe acute respiratory illness.

Materials and methods: retrospective collection of data records and previously collected nasopharyngeal and oropharyngeal sample stored in our lab for determining the start of COVID-19 in our hospitals, using RT-PCR and postive samples we will conduct Sequencing analysis and photogenic analysis. Also epidemiological and data study of the negatively screened patients, to determine the most common causes of SARI and the causes of mortality in this category of patients

Keywords

COVID-19, EGYPT, SARI

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Method Article

Sara H.A.Agwa, Hesham Elghazaly, Sarah El-Nakeep, Ahmad Moustafa, Manal H El-Sayed, Hala Hafez, Samia abdo, Marwa Matboli, Maha Saad, Shaimaa M.Elsayed, Aya M.Abd Elsamie, Reham M.Darwish, Hoda ezz elarab, Fatma S. E. Ebeid, Sara Makkeyah, Mahmoud S El Meteini

Sara H.A.Agwa

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Hesham Elghazaly

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Sarah El-Nakeep

Department of Internal Medicine, Ain Shams University

Corresponding Author

Ahmad Moustafa

Department of Biology, American University in Cairo, New Cairo, Egypt

Manal H El-Sayed

• Pediatric Hematology Oncology Department, Ain Shams University

Hala Hafez

Clinical pathology Department, Ain Shams University

Samia abdo

Clinical pathology Department, Ain Shams University

Marwa Matboli

Biochemistry and molecular biology Department, Ain Shams University

Maha Saad

Medicinal biochemistry and molecular biology Department, Modern University for Technology and Information

Shaimaa M.Elsayed

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Aya M.Abd Elsamie

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Reham M.Darwish

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Hoda ezz elarab

Clinical pathology Department, Ain Shams University

Fatma S. E. Ebeid

• Pediatric Hematology Oncology Department, Ain Shams University

Sara Makkeyah

Pediatric Hematology Oncology Department, Ain Shams University

Mahmoud S El Meteini

Department of General Surgery, Ain Shams University

Version History

CURRENT STATUS: Posted

Version 1

Posted 24 Sep, 2020

Community comments: 1

MetricsComments: 0HTML Views: ...

DOI:

10.21203/rs.3.pex-1157/v1

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License:

This work is licensed under a CC BY 4.0 License. Read Full License

Aim: to know the start of COVID-19 in Ain shams University hospitals (El-Demerdash) in Severe acute respiratory illness.

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Sara H.A.Agwa, Hesham Elghazaly, Sarah El-Nakeep, Ahmad Moustafa, Manal H El-Sayed, Hala Hafez, Samia abdo, Marwa Matboli, Maha Saad, Shaimaa M.Elsayed, Aya M.Abd Elsamie, Reham M.Darwish, Hoda ezz elarab, Fatma S. E. Ebeid, Sara Makkeyah, Mahmoud S El Meteini

Sara H.A.Agwa

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Hesham Elghazaly

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Sarah El-Nakeep

Department of Internal Medicine, Ain Shams University

Corresponding Author

Ahmad Moustafa

Department of Biology, American University in Cairo, New Cairo, Egypt

Manal H El-Sayed

• Pediatric Hematology Oncology Department, Ain Shams University

Hala Hafez

Clinical pathology Department, Ain Shams University

Samia abdo

Clinical pathology Department, Ain Shams University

Marwa Matboli

Biochemistry and molecular biology Department, Ain Shams University

Maha Saad

Medicinal biochemistry and molecular biology Department, Modern University for Technology and Information

Shaimaa M.Elsayed

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Aya M.Abd Elsamie

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Reham M.Darwish

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Hoda ezz elarab

Clinical pathology Department, Ain Shams University

Fatma S. E. Ebeid

• Pediatric Hematology Oncology Department, Ain Shams University

Sara Makkeyah

Pediatric Hematology Oncology Department, Ain Shams University

Mahmoud S El Meteini

Department of General Surgery, Ain Shams University

DOI:

10.21203/rs.3.pex-1157/v1

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

COVID-19, EGYPT, SARI

Introduction

Reagents

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References

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Subject Areas

Computational biology and bioinformatics

Molecular biology

Genetics

An open repository of community-contributed protocols sponsored by Nature Research.

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Method Article

Sara H.A.Agwa, Hesham Elghazaly, Sarah El-Nakeep, Ahmad Moustafa, Manal H El-Sayed, Hala Hafez, Samia abdo, Marwa Matboli, Maha Saad, Shaimaa M.Elsayed, Aya M.Abd Elsamie, Reham M.Darwish, Hoda ezz elarab, Fatma S. E. Ebeid, Sara Makkeyah, Mahmoud S El Meteini

Sara H.A.Agwa

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Hesham Elghazaly

Faculty of Medicine Ain Shams Research Institute-Molecular Genomic Unit (MASRI-Molecular Genomic Unit), Ain Shams University

Sarah El-Nakeep

Department of Internal Medicine, Ain Shams University

Corresponding Author

Ahmad Moustafa

Department of Biology, American University in Cairo, New Cairo, Egypt

Manal H El-Sayed

• Pediatric Hematology Oncology Department, Ain Shams University

Hala Hafez

Clinical pathology Department, Ain Shams University