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Method Article
Methodologies and Procedures Employed in the Identification and Quantitation of Lipid Mediators via LC-MS/MS
Jesmond Dalli
William Harvey Research Institute, Queen Mary University of London
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6328-3640
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
Liquid chromatography tandem mass spectrometry (LC-MS/MS) is a sensitive and robust approach for the identification and quantitation of lipid mediators including the specialized pro-resolving mediators (resolvins, maresins, protectins and lipoxins), and the classic eicosanoids. Due to the relatively low concentration of these molecules in biological samples, this methodology is classically coupled with solid phase extraction (SPE) techniques that enhance the sensitivity of the assay. Herein, we provide a step-by-step description of instrumentation employed in the both SPE and LC-MS/MS and detailed methodologies employed in the extraction of these lipid mediators and their identification and quantitation using LC-MS/MS.
Keywords
Lipid mediator profiling, flux analysis, eicosanoids, resolvins, protectins, maresins, SPE, liquid chromatography-tandem mass spectrometry.
Figure 1
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Tables 1-6
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Method Article
Methodologies and Procedures Employed in the Identification and Quantitation of Lipid Mediators via LC-MS/MS
Jesmond Dalli
William Harvey Research Institute, Queen Mary University of London
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6328-3640
Version History
CURRENT STATUS: Posted
Version 1
Posted 28 Oct, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
Liquid chromatography tandem mass spectrometry (LC-MS/MS) is a sensitive and robust approach for the identification and quantitation of lipid mediators including the specialized pro-resolving mediators (resolvins, maresins, protectins and lipoxins), and the classic eicosanoids. Due to the relatively low concentration of these molecules in biological samples, this methodology is classically coupled with solid phase extraction (SPE) techniques that enhance the sensitivity of the assay. Herein, we provide a step-by-step description of instrumentation employed in the both SPE and LC-MS/MS and detailed methodologies employed in the extraction of these lipid mediators and their identification and quantitation using LC-MS/MS.
Figure 1