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Method Article
Jiyoung Lee
Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7717-8104
Fumitoshi Ishino
Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8458-6069
License:
This work is licensed under a CC BY 4.0 License. Read Full License
For the understanding of the spatiotemporal regulation of cardiogenesis, it is important to generate in vitro model of hearts. This protocol introduces how to generate heart organoids from mouse embryonic stem cell-derived embryoid bodies (EB) in the presence of the laminin-entactin (LN/ET) complex and fibroblast growth factor 4 (FGF4) by three consecutive steps, 1) the culture of ESCs, 2) in vitro differentiation of ESCs into EBs and 3) in vitro culture of EBs for the generation of heart organoids. The generated heart organoids possess structural and functional capacity similar to atrial and ventricular parts of in vivo embryonic heart. This simplified protocol also provides a promising research tool with a broad range of applications, including drug testing.
Keywords
Mouse, heart development, organoids, extracellular matrix, embryonic stem cells
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An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Jiyoung Lee
Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-7717-8104
Fumitoshi Ishino
Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University
Corresponding Author
ORCiD: https://orcid.org/0000-0001-8458-6069
Version History
CURRENT STATUS: Posted
Version 1
Posted 03 Sep, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
For the understanding of the spatiotemporal regulation of cardiogenesis, it is important to generate in vitro model of hearts. This protocol introduces how to generate heart organoids from mouse embryonic stem cell-derived embryoid bodies (EB) in the presence of the laminin-entactin (LN/ET) complex and fibroblast growth factor 4 (FGF4) by three consecutive steps, 1) the culture of ESCs, 2) in vitro differentiation of ESCs into EBs and 3) in vitro culture of EBs for the generation of heart organoids. The generated heart organoids possess structural and functional capacity similar to atrial and ventricular parts of in vivo embryonic heart. This simplified protocol also provides a promising research tool with a broad range of applications, including drug testing.
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