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Method Article
Sample preparation and imaging procedures for fast and multiplexed superresolution microscopy with DNA-PAINT-ERS
Xiaolin Nan
Oregon Health and Science University
Corresponding Author
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DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis.
Keywords
superresolution microscopy, single-molecule localization microscopy, DNA-PAINT, multiplexed imaging, antibody oligonucleotide conjugation, immunofluorescence, DNA hybridization
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This is a preprint. It has not completed peer review.
Method Article
Sample preparation and imaging procedures for fast and multiplexed superresolution microscopy with DNA-PAINT-ERS
Xiaolin Nan
Oregon Health and Science University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 01 Sep, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) facilitates multiplexing in superresolution microscopy but is practically limited by slow imaging speed. We have developed DNA-PAINT-ERS, where E=ethylene carbonate, R=repeating sequence, and S=spacer, for fast and multiplexed superresolution imaging with DNA-PAINT. Here we describe detailed procedures for DNA-PAINT-ERS including reagent preparation, sample labeling, as well as image acqusition and analysis.